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Journal of Biomedicine and Biotechnology
Volume 2004 (2004), Issue 4, Pages 195-202
http://dx.doi.org/10.1155/S1110724304401016
Research article

Expression of Mn-Superoxide Dismutase Gene in Nontumorigenic and Tumorigenic Human Mammary Epithelial Cells

1Department of Pharmacology, Meharry Medical College, Nashville, TN 37208, USA
2Department of Biochemistry, Meharry Medical College, Nashville, TN 37208, USA

Received 13 January 2004; Revised 26 March 2004; Accepted 17 April 2004

Copyright © 2004. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Manganese superoxide dismutase (Mn-SOD), localized at the mitochondrial matrix, has the ability to protect cells against oxidative damage. It has been reported that low levels of Mn-SOD gene expression cause the development of certain kind of tumors. On the other hand, overexpression of Mn-SOD gene may play an important role in the development of cancer. In our study, we find that Mn-SOD activity was higher in nonaggressive (MCF-7) and aggressive (BT-549 and 11-9-14) breast cancer cell lines compared to that of nontumorigenic (MCF-12A and MCF-12F) mammary epithelial cell lines. We also observed an increased expression of Mn-SOD gene in cancerous cell lines. The elevated level of SOD activity in nonaggressive and aggressive breast epithelial cell lines was associated with some changes in nucleotide sequence.