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Journal of Biomedicine and Biotechnology
Volume 2005 (2005), Issue 3, Pages 254-270
http://dx.doi.org/10.1155/JBB.2005.254
Research article

Identification, Structural, and Functional Characterization of a New Early Gene (6A3-5, 7 kb): Implication in the Proliferation and Differentiation of Smooth Muscle Cells

1INSERM XR331, Faculty of Medicine RTH Laënnec, Lyon 69372, France
2Genomics and Atherothrombosis Laboratory, Thrombosis Research Institute, London SW3 6LR, UK
3Center for Cardiovascular Biology and Medicine, King's College, University of London, London WC2R 2LS, UK
4Medical Oncology Laboratory, Queen Mary & St. Bartholomew's Medical School, Cancer Research UK, Charterhouse Square, London EC1M 6BQ, UK

Received 9 December 2004; Revised 3 March 2005; Accepted 21 March 2005

Copyright © 2005 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Arterial smooth muscle cells (SMCs) play a major role in atherosclerosis and restenosis. Differential display was used to compare transcription profiles of synthetic SMCs to proliferating rat cultured SMC line. An isolated cDNA band (6A3-5) was shown by northern (7 kb) to be upregulated in the proliferating cell line. A rat tissue northern showed differential expression of this gene in different tissues. Using 5’ RACE and screening of a rat brain library, part of the cDNA was cloned and sequenced (5.4 kb). Sequence searches showed important similarities with a new family of transcription factors, bearing ARID motifs. A polyclonal antibody was raised and showed a protein band of 175 kd, which is localized intracellularly. We also showed that 6A3-5 is upregulated in dedifferentiated SMC (P9) in comparison to contractile SMC ex vivo (P0). This work describes cloning, structural, and functional characterization of a new early gene involved in SMC phenotype modulation.