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Journal of Biomedicine and Biotechnology
Volume 2005 (2005), Issue 2, Pages 87-95
http://dx.doi.org/10.1155/JBB.2005.87
Research article

Objective Clustering of Proteins Based on Subcellular Location Patterns

1Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA
2Department of Biomedical Engineering, 2100 Doherty Hall, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, PA 15213-3890, USA
3Center for Automated Learning and Discovery, School of Computer Science, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, PA 15213-3891, USA

Received 8 September 2004; Accepted 4 November 2004

Copyright © 2005 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The goal of proteomics is the complete characterization of all proteins. Efforts to characterize subcellular location have been limited to assigning proteins to general categories of organelles. We have previously designed numerical features to describe location patterns in microscope images and developed automated classifiers that distinguish major subcellular patterns with high accuracy (including patterns not distinguishable by visual examination). The results suggest the feasibility of automatically determining which proteins share a single location pattern in a given cell type. We describe an automated method that selects the best feature set to describe images for a given collection of proteins and constructs an effective partitioning of the proteins by location. An example for a limited protein set is presented. As additional data become available, this approach can produce for the first time an objective systematics for protein location and provide an important starting point for discovering sequence motifs that determine localization.