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Journal of Biomedicine and Biotechnology
Volume 2006 (2006), Article ID 49193, 8 pages
http://dx.doi.org/10.1155/JBB/2006/49193
Research Article

Reduced Atherosclerotic Lesion Size in P-Selectin Deficient Apolipoprotein E-Knockout Mice Fed a Chow but Not a Fat Diet

1EA-3740, INSERM-IFR62, Faculté de Médecine Laënnec, Université Claude Bernard Lyon 1, 7 rue Guillaume Paradin, Lyon 69372, France
2Centre for Cardiovascular Biology and Medicine, Guy's Campus, King's College London, London SE1 1UL, United Kingdom
3INSERM Unit 689, Hospital Lariboisiére, Paris, France
4Genomics and Atherothrombosis Laboratory, Thrombosis Research Institute, London SW3 6LR, United Kingdom

Received 15 September 2005; Revised 27 December 2005; Accepted 29 December 2005

Copyright © 2006 Marie-Claude Bourdillon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Endothelial cells lining atherosclerotic, but not healthy sites, on human arteries express P-selectin. We investigated the role of P-selectin on the development of vascular lesions in an ApoE/ male mice. Double-knockout (ApoE/, P-selectin-/-; DKO) were compared to single-knockout (ApoE/; SKO) mice. They were fed a chow or fat diet for 3, 6, 15, and 20 weeks, without any differences in cholesterol levels. DKO mice fed a chow diet exhibited a ratio of lesion area over media lower than SKO mice, for 3 (P<.03) , 6 (P<.001), and 15 (P<.02) weeks. DKO mice fed a fat diet showed a lower ratio only at 3 weeks. P-selectin deficiency in ApoE/ mice has a protective effect in atherosclerotic lesions development. Reduction of lesion size depends on diet type and duration. A fat diet could neutralize the beneficial effects of P-selectin deficiency, inducing atherosclerotic lesions via probably other adhesion molecules.