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Journal of Biomedicine and Biotechnology
Volume 2006 (2006), Article ID 65052, 8 pages
http://dx.doi.org/10.1155/JBB/2006/65052
Review Article

Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi

1Department of Biophysics and Biochemistry, Graduate School of Science and Undergraduate Program for Bioinformatics and Systems Biology, School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
2Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

Received 25 January 2006; Accepted 17 April 2006

Copyright © 2006 Kumiko Ui-Tei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Short interfering RNAs (siRNAs) are widely used to bring about RNA interference (RNAi) in mammalian cells. Numerous siRNAs may be designed for any target gene though most of which would be incapable of efficiently inducing mammalian RNAi. Certain highly functional siRNAs designed for knockout of a particular gene may render unrelated endogenous genes nonfunctional. These major bottlenecks should be properly eliminated when RNAi technologies are employed for any experiment in mammalian functional genomics. This paper thus presents essential notes and findings regarding the proper choice of siRNA-sequence selection algorithms and web-based online software systems.