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Journal of Biomedicine and Biotechnology
Volume 2006 (2006), Article ID 87340, 13 pages
http://dx.doi.org/10.1155/JBB/2006/87340
Review Article

RNA-Mediated Gene Silencing in Hematopoietic Cells

Department of Hematology, Hemostasis, and Oncology, Hannover Medical School, Hannover 30625, Germany

Received 8 February 2006; Accepted 3 April 2006

Copyright © 2006 Letizia Venturini et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the past few years, the discovery of RNA-mediated gene silencing mechanisms, like RNA interference (RNAi), has revolutionized our understanding of eukaryotic gene expression. These mechanisms are activated by double-stranded RNA (dsRNA) and mediate gene silencing either by inducing the sequence-specific degradation of complementary mRNA or by inhibiting mRNA translation. RNAi now provides a powerful experimental tool to elucidate gene function in vitro and in vivo, thereby opening new exciting perspectives in the fields of molecular analysis and eventually therapy of several diseases such as infections and cancer. In hematology, numerous studies have described the successful application of RNAi to better define the role of oncogenic fusion proteins in leukemogenesis and to explore therapeutic approaches in hematological malignancies. In this review, we highlight recent advances and caveats relating to the application of this powerful new methodology to hematopoiesis.