Distinction between Pore Assembly by Staphylococcal <mml:math id="E1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>α</mml:mi></mml:math>-Toxin versus Leukotoxins

Joubert, Olivier; Voegelin, Joëlle; Guillet, Valérie; Tranier, Samuel; Werner, Sandra; Colin, Didier A.; Dalla Serra, Mauro; Keller, Daniel; Monteil, Henri; Mourey, Lionel; Prévost, Gilles

doi:https://doi.org/10.1155/2007/25935

BioMed Research International

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Research Article | Open Access

Volume 2007 | Article ID 025935 | https://doi.org/10.1155/2007/25935

Distinction between Pore Assembly by Staphylococcal α-Toxin versus Leukotoxins

Olivier Joubert,¹Joëlle Voegelin,¹Valérie Guillet,²Samuel Tranier,²Sandra Werner,³Didier A. Colin,¹Mauro Dalla Serra,⁴Daniel Keller,¹Henri Monteil,¹Lionel Mourey,²and Gilles Prévost¹

Academic Editor: Shahid Jameel

Received10 Aug 2006

Revised07 Nov 2006

Accepted06 Dec 2006

Published28 Feb 2007

Abstract

The staphylococcal bipartite leukotoxins and the homoheptameric α-toxin belong to the same family of β-barrel pore-forming toxins despite slight differences. In the α-toxin pore, the N-terminal extremity of each protomer interacts as a deployed latch with two consecutive protomers in the vicinity of the pore lumen. N-terminal extremities of leukotoxins as seen in their three-dimensional structures are heterogeneous in length and take part in the β-sandwich core of soluble monomers. Hence, the interaction of these N-terminal extremities within structures of adjacent monomers is questionable. We show here that modifications of their N-termini by two different processes, using fusion with glutathione S-transferase (GST) and bridging of the N-terminal extremity to the adjacent β-sheet via disulphide bridges, are not deleterious for biological activity. Therefore, bipartite leukotoxins do not need a large extension of their N-terminal extremities to form functional pores, thus illustrating a microheterogeneity of the structural organizations between bipartite leukotoxins and α-toxin.

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Copyright © 2007 Olivier Joubert et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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