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Journal of Biomedicine and Biotechnology
Volume 2007 (2007), Article ID 26065, 10 pages
http://dx.doi.org/10.1155/2007/26065
Research Article

The Many Facets of SDF-1α, CXCR4 Agonists and Antagonists on Hematopoietic Progenitor Cells

1Department of Medicine V, University of Heidelberg, Im Neuenheimer Feld 410, Heidelberg 69120, Germany
2Chemokine Therapeutics Corporation, 6190 Agronomy Road, Vancouver, BC, Canada V6T 1Z3
3AnorMED, 20353 64th Avenue, Langley, BC, Canada V2Y 1N5
4Department of Physiology and Pathophysiology, University of Heidelberg, Im Neuenheimer Feld 326, Heidelberg 69120, Germany

Received 20 September 2006; Revised 4 January 2007; Accepted 14 February 2007

Academic Editor: James L. Sherley

Copyright © 2007 Anne Faber et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Stromal cell-derived factor-1alpha (SDF-1α) has pleiotropic effects on hematopoietic progenitor cells (HPCs). We have monitored podia formation, migration, proliferation, and cell-cell adhesion of human HPC under the influence of SDF-1α, a peptide agonist of CXCR4 (CTCE-0214), a peptide antagonist (CTCE-9908), and a nonpeptide antagonist (AMD3100). Whereas SDF-1α induced migration of CD34+ cells in a dose-dependent manner, CTCE-0214, CTCE-9908, and AMD3100 did not induce chemotaxis in this concentration range albeit the peptides CTCE-0214 and CTCE-9908 increased podia formation. Cell-cell adhesion of HPC to human mesenchymal stromal cells was impaired by the addition of SDF-1α, CTCE-0214, and AMD3100. Proliferation was not affected by SDF-1α or its analogs. Surface antigen detection of CXCR4 was reduced upon treatment with SDF-1α or AMD3100 and it was enhanced by CTCE-9908. Despite the fact that all these molecules target the same CXCR4 receptor, CXCR4 agonists and antagonists have selective effects on different functions of the natural molecule.