The Many Facets of SDF-1<mml:math id="E1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>α</mml:mi></mml:math>, CXCR4 Agonists and Antagonists on Hematopoietic Progenitor Cells

Faber, Anne; Roderburg, Christoph; Wein, Frederik; Saffrich, Rainer; Seckinger, Anja; Horsch, Kerstin; Diehlmann, Anke; Wong, Donald; Bridger, Gary; Eckstein, Volker; Ho, Anthony D.; Wagner, Wolfgang

doi:https://doi.org/10.1155/2007/26065

BioMed Research International

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Research Article | Open Access

Volume 2007 | Article ID 026065 | https://doi.org/10.1155/2007/26065

The Many Facets of SDF-1α, CXCR4 Agonists and Antagonists on Hematopoietic Progenitor Cells

Anne Faber,¹Christoph Roderburg,¹Frederik Wein,¹Rainer Saffrich,¹Anja Seckinger,¹Kerstin Horsch,¹Anke Diehlmann,¹Donald Wong,²Gary Bridger,³Volker Eckstein,¹Anthony D. Ho,¹and Wolfgang Wagner^1,4

Academic Editor: James L. Sherley

Received20 Sept 2006

Revised04 Jan 2007

Accepted14 Feb 2007

Published23 Apr 2007

Abstract

Stromal cell-derived factor-1alpha (SDF-1α) has pleiotropic effects on hematopoietic progenitor cells (HPCs). We have monitored podia formation, migration, proliferation, and cell-cell adhesion of human HPC under the influence of SDF-1α, a peptide agonist of CXCR4 (CTCE-0214), a peptide antagonist (CTCE-9908), and a nonpeptide antagonist (AMD3100). Whereas SDF-1α induced migration of CD34+ cells in a dose-dependent manner, CTCE-0214, CTCE-9908, and AMD3100 did not induce chemotaxis in this concentration range albeit the peptides CTCE-0214 and CTCE-9908 increased podia formation. Cell-cell adhesion of HPC to human mesenchymal stromal cells was impaired by the addition of SDF-1α, CTCE-0214, and AMD3100. Proliferation was not affected by SDF-1α or its analogs. Surface antigen detection of CXCR4 was reduced upon treatment with SDF-1α or AMD3100 and it was enhanced by CTCE-9908. Despite the fact that all these molecules target the same CXCR4 receptor, CXCR4 agonists and antagonists have selective effects on different functions of the natural molecule.

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Copyright © 2007 Anne Faber et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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