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Journal of Biomedicine and Biotechnology
Volume 2007 (2007), Article ID 69036, 19 pages
http://dx.doi.org/10.1155/2007/69036
Review Article

Significance of Nano- and Microtopography for Cell-Surface Interactions in Orthopaedic Implants

1Department of Orthopaedics, Heinrich-Heine University Medical School, Moorenstrasse 5, Duesseldorf 40225, Germany
2Institute of Anatomy II, Heinrich-Heine University Medical School, Universitätsstrasse 1, Duesseldorf 40225, Germany

Received 18 March 2007; Accepted 5 August 2007

Academic Editor: Hicham Fenniri

Copyright © 2007 M. Jäger et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. B. C. Ward and T. J. Webster, “The effect of nanotopography on calcium and phosphorus deposition on metallic materials in vitro,” Biomaterials, vol. 27, no. 16, pp. 3064–3074, 2006. View at Publisher · View at Google Scholar · View at PubMed
  2. C.-Y. Lai, B. G. Trewyn, and D. M. Jeftinija, et al., “A mesoporous silica nanosphere-based carrier system with chemically removable CdS nanoparticle caps for stimuli-responsive controlled release of neurotransmitters and drug molecules,” Journal of the American Chemical Society, vol. 125, no. 15, pp. 4451–4459, 2003. View at Publisher · View at Google Scholar · View at PubMed
  3. A. P. Alivisatos, W. Gu, and C. Larabell, “Quantum dots as cellular probes,” Annual Review of Biomedical Engineering, vol. 7, pp. 55–76, 2005. View at Publisher · View at Google Scholar · View at PubMed
  4. R. M. Gul, F. J. McGarry, C. R. Bragdon, O. K. Muratoglu, and W. H. Harris, “Effect of consolidation on adhesive and abrasive wear of ultra high molecular weight polyethylene,” Biomaterials, vol. 24, no. 19, pp. 3193–3199, 2003. View at Publisher · View at Google Scholar
  5. D. Di Iorio, T. Traini, M. Degidi, S. Caputi, J. Neugebauer, and A. Piattelli, “Quantitative evaluation of the fibrin clot extension on diffferent implant surfaces: an in vitro study,” Journal of Biomedical Materials Research Part B, vol. 74, no. 1, pp. 636–642, 2005. View at Publisher · View at Google Scholar · View at PubMed
  6. K.-I. Inoue, H. Takano, and R. Yanagisawa, et al., “Effects of nano particles on antigen-related airway inflammation in mice,” Respiratory Research, vol. 6, p. 106, 2005. View at Publisher · View at Google Scholar · View at PubMed
  7. D. Kim, H. El-Shall, D. Dennis, and T. Morey, “Interaction of PLGA nanoparticles with human blood constituents,” Colloids and Surfaces B, vol. 40, no. 2, pp. 83–91, 2005. View at Publisher · View at Google Scholar · View at PubMed
  8. D. O. Meredith, L. Eschbach, M. O. Riehle, A. S. G. Curtis, and R. G. Richards, “Microtopography of metal surfaces influence fibroblast growth by modifying cell shape, cytoskeleton, and adhesion,” Journal of Orthopaedic Research, 2007. View at Publisher · View at Google Scholar · View at PubMed
  9. R. L. Jilka, R. S. Weinstein, T. Bellido, A. M. Parfitt, and S. C. Manolagas, “Osteoblast programmed cell death (apoptosis): modulation by growth factors and cytokines,” Journal of Bone and Mineral Research, vol. 13, no. 5, pp. 793–802, 1998. View at Publisher · View at Google Scholar · View at PubMed
  10. C. Schmidt, D. Kaspar, M. R. Sarkar, L. E. Claes, and A. A. Ignatius, “A scanning electron microscopy study of human osteoblast morphology on five orthopedic metals,” Journal of Biomedical Materials Research, vol. 63, no. 3, pp. 252–261, 2002. View at Publisher · View at Google Scholar · View at PubMed
  11. K. Anselme and M. Bigerelle, “Topography effects of pure titanium substrates on human osteoblast long-term adhesion,” Acta Biomaterialia, vol. 1, no. 2, pp. 211–222, 2005. View at Publisher · View at Google Scholar · View at PubMed
  12. K. Anselme and M. Bigerelle, “Statistical demonstration of the relative effect of surface chemistry and roughness on human osteoblast short-term adhesion,” Journal of Materials Science: Materials in Medicine, vol. 17, no. 5, pp. 471–479, 2006. View at Publisher · View at Google Scholar · View at PubMed
  13. K. Anselme, B. Noël, and P. Hardouin, “Human osteoblast adhesion on titanium alloy, stainless steel, glass and plastic substrates with same surface topography,” Journal of Materials Science: Materials in Medicine, vol. 10, no. 12, pp. 815–819, 1999. View at Publisher · View at Google Scholar
  14. M. Bigerelle and K. Anselme, “Statistical correlation between cell adhesion and proliferation on biocompatible metallic materials,” Journal of Biomedical Materials Research Part A, vol. 72, no. 1, pp. 36–46, 2005. View at Publisher · View at Google Scholar · View at PubMed
  15. M. Bigerelle and K. Anselme, “A kinetic approach to osteoblast adhesion on biomaterial surface,” Journal of Biomedical Materials Research Part A, vol. 75, no. 3, pp. 530–540, 2005. View at Publisher · View at Google Scholar · View at PubMed
  16. F. Pérez-Willard, D. Wolde-Giorgis, and T. Al-Kassab, et al., “Focused ion beam preparation of atom probe specimens containing a single crystallographically well-defined grain boundary,” to appear in Micron. View at Publisher · View at Google Scholar · View at PubMed
  17. U. Meyer, A. Büchter, H. P. Wiesmann, U. Joos, and D. B. Jones, “Basic reactions of osteoblasts on structured material surfaces,” European Cells & Materials, vol. 9, pp. 39–49, 2005.
  18. S. Teixeira, F. J. Monteiro, M. P. Ferraz, R. Vilar, and S. Eugeénio, “Laser surface treatment of hydroxyapatite for enhanced tissue integration: surface characterization and osteoblastic interaction studies,” Journal of Biomedical Materials Research Part A, vol. 81, no. 4, pp. 920–929, 2007. View at Publisher · View at Google Scholar · View at PubMed
  19. M. Bigerelle, K. Anselme, E. Dufresne, P. Hardouin, and A. Iost, “An unscaled parameter to measure the order of surfaces: a new surface elaboration to increase cells adhesion,” Biomolecular Engineering, vol. 19, no. 2–6, pp. 79–83, 2002. View at Publisher · View at Google Scholar
  20. K. Anselme, M. Bigerelle, I. Loison, B. Noël, and P. Hardouin, “Kinetic study of the expression of β-catenin, actin and vinculin during osteoblastic adhesion on grooved titanium substrates,” Bio-Medical Materials and Engineering, vol. 14, no. 4, pp. 545–556, 2004.
  21. T. J. Webster, C. Ergun, R. H. Doremus, R. W. Siegel, and R. Bizios, “Enhanced functions of osteoblasts on nanophase ceramics,” Biomaterials, vol. 21, no. 17, pp. 1803–1810, 2000. View at Publisher · View at Google Scholar
  22. F. Kaplan, W. Hayes, T. Keaveny, A. Boskey, T. Einhorn, and J. Iannotti, “Influence of titanium surfaces on attachment of osteoblast-like cells in vitro,” in Orthopedic Basic Science, S. Sp, Ed., pp. 460–478, American Academy of Orthopedic Surgeons, Columbus, Ohio, USA, 1994.
  23. F. Grizon, E. Aguado, G. Huré, M. F. Baslé, and D. Chappard, “Enhanced bone integration of implants with increased surface roughness: a long term study in the sheep,” Journal of Dentistry, vol. 30, no. 5-6, pp. 195–203, 2002. View at Publisher · View at Google Scholar
  24. T. W. Phillips and S. S. Messieh, “Cementless hip replacement for arthritis. Problems with a smooth surface Moore stem,” Journal of Bone and Joint Surgery, British, vol. 70, no. 5, pp. 750–755, 1988.
  25. M. M. Shalabi, A. Gortemaker, M. A. Van't Hof, J. A. Jansen, and N. H. J. Creugers, “Implant surface roughness and bone healing: a systematic review,” Journal of Dental Research, vol. 85, no. 6, pp. 496–500, 2006.
  26. M. Jäger, T. Fischer, A. Schultheis, S. Lensing-Höhn, and R. Krauspe, “Extensive H+ release by bone substitutes affects biocompatibility in vitro testing,” Journal of Biomedical Materials Research Part A, vol. 76, no. 2, pp. 310–322, 2006. View at Publisher · View at Google Scholar · View at PubMed
  27. M. Jäger and A. Wilke, “Comprehensive biocompatibility testing of a new PMMA-HA bone cement versus conventional PMMA cement in vitro,” Journal of Biomaterials Science, Polymer Edition, vol. 14, no. 11, pp. 1283–1298, 2003. View at Publisher · View at Google Scholar
  28. M. Jayaraman, U. Meyer, M. Bühner, U. Joos, and H.-P. Wiesmann, “Influence of titanium surfaces on attachment of osteoblast-like cells in vitro,” Biomaterials, vol. 25, no. 4, pp. 625–631, 2004. View at Publisher · View at Google Scholar
  29. J. Y. Martin, Z. Schwartz, and T. W. Hummert, et al., “Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63),” Journal of Biomedical Materials Research, vol. 29, no. 3, pp. 389–401, 1995. View at Publisher · View at Google Scholar · View at PubMed
  30. Z. Schwartz and B. D. Boyan, “Underlying mechanisms at the bone-biomaterial interface,” Journal of Cellular Biochemistry, vol. 56, no. 3, pp. 340–347, 1994. View at Publisher · View at Google Scholar · View at PubMed
  31. Z. Schwartz, K. Kieswetter, D. D. Dean, and B. D. Boyan, “Underlying mechanisms at the bone-surface interface during regeneration,” Journal of Periodontal Research, vol. 32, no. 1, part 2, pp. 166–171, 1997. View at Publisher · View at Google Scholar
  32. L. F. Cooper, “A role for surface topography in creating and maintaining bone at titanium endosseous implants,” Journal of Prosthetic Dentistry, vol. 84, no. 5, pp. 522–534, 2000. View at Publisher · View at Google Scholar · View at PubMed
  33. W. Macdonald, P. Campbell, J. Fisher, and A. Wennerberg, “Variation in surface texture measurements,” Journal of Biomedical Materials Research Part B, vol. 70, no. 2, pp. 262–269, 2004.
  34. J. D. Andrade and V. Hlady, “Plasma protein adsorption: the big twelve,” Annals of the New York Academy of Sciences, vol. 516, pp. 158–172, 1987. View at Publisher · View at Google Scholar
  35. M. Hakansson and S. Linse, “Protein reconstitution and 3D domain swapping,” Current Protein and Peptide Science, vol. 3, no. 6, pp. 629–642, 2002. View at Publisher · View at Google Scholar
  36. D. J. Iuliano, S. S. Saavedra, and G. A. Truskey, “Effect of the conformation and orientation of adsorbed fibronectin on endothelial cell spreading and the strength of adhesion,” Journal of Biomedical Materials Research, vol. 27, no. 8, pp. 1103–1113, 1993. View at Publisher · View at Google Scholar · View at PubMed
  37. J. G. Steele, B. A. Dalton, G. Johnson, and P. A. Underwood, “Adsorption of fibronectin and vitronectin onto PrimariaTM and tissue culture polystyrene and relationship to the mechanism of initial attachment of human vein endothelial cells and BHK-21 fibroblasts,” Biomaterials, vol. 16, no. 14, pp. 1057–1067, 1995. View at Publisher · View at Google Scholar
  38. L. Vroman, “The importance of surfaces in contact phase reactions,” Seminars in Thrombosis and Hemostasis, vol. 13, no. 1, pp. 79–85, 1987.
  39. M. Balcerzak, E. Hamade, and L. Zhang, et al., “The roles of annexins and alkaline phosphatase in mineralization process,” Acta Biochimica Polonica, vol. 50, no. 4, pp. 1019–1038, 2003.
  40. F. M. Pavalko, S. M. Norvell, D. B. Burr, C. H. Turner, R. L. Duncan, and J. P. Bidwell, “A model for mechanotransduction in bone cells: the load-bearing mechanosomes,” Journal of Cellular Biochemistry, vol. 88, no. 1, pp. 104–112, 2003. View at Publisher · View at Google Scholar · View at PubMed
  41. F. A. Weyts, B. Bosmans, R. Niesing, J. P. van Leeuwen, and H. Weinans, “Mechanical control of human osteoblast apoptosis and proliferation in relation to differentiation,” Calcified Tissue International, vol. 72, no. 4, pp. 505–512, 2003. View at Publisher · View at Google Scholar · View at PubMed
  42. D. E. Ingber, “Mechanobiology and diseases of mechanotransduction,” Annals of Medicine, vol. 35, no. 8, pp. 564–577, 2003. View at Publisher · View at Google Scholar
  43. M. G. Coppolino and S. Dedhar, “Bi-directional signal transduction by integrin receptors,” The International Journal of Biochemistry and Cell Biology, vol. 32, no. 2, pp. 171–188, 2000. View at Publisher · View at Google Scholar
  44. F. G. Giancotti and E. Ruoslahti, “Integrin signaling,” Science, vol. 285, no. 5430, pp. 1028–1032, 1999. View at Publisher · View at Google Scholar
  45. A. Howe, A. E. Aplin, S. K. Alahari, and R. Juliano, “Integrin signaling and cell growth control,” Current Opinion in Cell Biology, vol. 10, no. 2, pp. 220–231, 1998. View at Publisher · View at Google Scholar
  46. U. Meyer, T. Meyer, and D. B. Jones, “Attachment kinetics, proliferation rates and vinculin assembly of bovine osteoblasts cultured on different pre-coated artificial substrates,” Journal of Materials Science: Materials in Medicine, vol. 9, no. 6, pp. 301–307, 1998. View at Publisher · View at Google Scholar
  47. A. M. Moursi, R. K. Globus, and C. H. Damsky, “Interactions between integrin receptors and fibronectin are required for calvarial osteoblast differentiation in vitro,” Journal of Cell Science, vol. 110, no. 18, pp. 2187–2196, 1997.
  48. A. Rezania and K. E. Healy, “The effect of peptide surface density on mineralization of a matrix deposited by osteogenic cells,” Journal of Biomedical Materials Research, vol. 52, no. 4, pp. 595–600, 2000. View at Publisher · View at Google Scholar
  49. B. D. Boyan, T. W. Hummert, D. D. Dean, and Z. Schwartz, “Role of material surfaces in regulating bone and cartilage cell response,” Biomaterials, vol. 17, no. 2, pp. 137–146, 1996. View at Publisher · View at Google Scholar
  50. M. Rouahi, E. Champion, O. Gallet, A. Jada, and K. Anselme, “Physico-chemical characteristics and protein adsorption potential of hydroxyapatite particles: influence on in vitro biocompatibility of ceramics after sintering,” Colloids and Surfaces B, vol. 47, no. 1, pp. 10–19, 2006. View at Publisher · View at Google Scholar · View at PubMed
  51. K. Webb, V. Hlady, and P. A. Tresco, “Relationships among cell attachment, spreading, cytoskeletal organization, and migration rate for anchorage-dependent cells on model surfaces,” Journal of Biomedical Materials Research, vol. 49, no. 3, pp. 362–368, 2000. View at Publisher · View at Google Scholar
  52. H. Schweikl, R. Müller, and C. Englert, et al., “Proliferation of osteoblasts and fibroblasts on model surfaces of varying roughness and surface chemistry,” Journal of Materials Science: Materials in Medicine, 2007. View at Publisher · View at Google Scholar · View at PubMed
  53. A. R. El-Ghannam, P. Ducheyne, and M. Risbud, et al., “Model surfaces engineered with nanoscale roughness and RGD tripeptides promote osteoblast activity,” Journal of Biomedical Materials Research Part A, vol. 68, no. 4, pp. 615–627, 2004. View at Publisher · View at Google Scholar · View at PubMed
  54. J. D. Andrade, “Interfacial phenomena and biomaterials,” Medical Instrumentation, vol. 7, no. 2, pp. 110–119, 1973.
  55. H. Heide, D. B. Jones, U. Meyer, K. Möller, B. Priessnitz, and D. H. Szulczewski, “The influence of zeta-potential and interfacial-tension on osteoblast-like cells,” Cells and Materials, vol. 4, no. 3, pp. 263–274, 1994.
  56. Y. J. Lim and Y. Oshida, “Initial contact angle measurements on variously treated dental/medical titanium materials,” Bio-Medical Materials and Engineering, vol. 11, no. 4, pp. 325–341, 2001.
  57. M. Jäger, F. Urselmann, and F. Witte, et al., “Osteoblast differentiation onto different biometals with a endoprosthetic surface geometry in vitro,” to appear in Journal of Biomedical Materials Research Part A. View at Publisher · View at Google Scholar · View at PubMed
  58. S. A. Redey, M. Nardin, and D. Bernache-Assolant, et al., “Behavior of human osteoblastic cells on stoichiometric hydroxyapatite and type A carbonate apatite: role of surface energy,” Journal of Biomedical Materials Research, vol. 50, no. 3, pp. 353–364, 2000. View at Publisher · View at Google Scholar
  59. G. Zhao, Z. Schwartz, and M. Wieland, et al., “High surface energy enhances cell response to titanium substrate microstructure,” Journal of Biomedical Materials Research Part A, vol. 74, no. 1, pp. 49–58, 2005. View at Publisher · View at Google Scholar · View at PubMed
  60. C. Stock, Y. Yang, and J. Ong, “Protein adsorption and cell adhesion on different implant metals,” in The IADR/AADR/CADR 83rd General Session, Baltimore, Md, USA, March 2005.
  61. J. D. Bumgardner, R. Wiser, S. H. Elder, R. Jouett, Y. Yang, and J. L. Ong, “Contact angle, protein adsorption and osteoblast precursor cell attachment to chitosan coatings bonded to titanium,” Journal of Biomaterials Science, Polymer Edition, vol. 14, no. 12, pp. 1401–1409, 2003. View at Publisher · View at Google Scholar
  62. Z. Qu, X. Rausch-Fan, M. Wieland, M. Matejka, and A. Schedle, “The initial attachment and subsequent behavior regulation of osteoblasts by dental implant surface modification,” Journal of Biomedical Materials Research Part A, vol. 82, no. 3, pp. 658–668, 2007. View at Publisher · View at Google Scholar · View at PubMed
  63. T. Kern, Y. Yang, R. Glover, and J. L. Ong, “Effect of heat-treated titanium surfaces on protein adsorption and osteoblast precursor cell initial attachment,” Implant Dentistry, vol. 14, no. 1, pp. 70–76, 2005. View at Publisher · View at Google Scholar
  64. D. E. MacDonald, B. E. Rapuano, N. Deo, M. Stranick, P. Somasundaran, and A. L. Boskey, “Thermal and chemical modification of titanium-aluminum-vanadium implant materials: effects on surface properties, glycoprotein adsorption, and MG63 cell attachment,” Biomaterials, vol. 25, no. 16, pp. 3135–3146, 2004. View at Publisher · View at Google Scholar · View at PubMed
  65. A. J. García and D. Boettiger, “Integrin-fibronectin interactions at the cell-material interface: initial integrin binding and signaling,” Biomaterials, vol. 20, no. 23-24, pp. 2427–2433, 1999. View at Publisher · View at Google Scholar
  66. P. T. de Oliveira and A. Nanci, “Nanotexturing of titanium-based surfaces upregulates expression of bone sialoprotein and osteopontin by cultured osteogenic cells,” Biomaterials, vol. 25, no. 3, pp. 403–413, 2004. View at Publisher · View at Google Scholar
  67. T. J. Webster and J. U. Ejiofor, “Increased osteoblast adhesion on nanophase metals: Ti, Ti6Al4V, and CoCrMo,” Biomaterials, vol. 25, no. 19, pp. 4731–4739, 2004. View at Publisher · View at Google Scholar · View at PubMed
  68. K. Das, S. Bose, and A. Bandyopadhyay, “Surface modifications and cell-materials interactions with anodized Ti,” Acta Biomaterialia, vol. 3, no. 4, pp. 573–585, 2007. View at Publisher · View at Google Scholar · View at PubMed
  69. K. H. Kim, T. Y. Kwon, and S. Y. Kim, et al., “Preparation and characterization of anodized titanium surfaces and their effect on osteoblast responses,” The Journal of Oral Implantology, vol. 32, no. 1, pp. 8–13, 2006. View at Publisher · View at Google Scholar
  70. J.-M. Park, J.-Y. Koak, J.-H. Jang, C.-H. Han, S.-K. Kim, and S.-J. Heo, “Osseointegration of anodized titanium implants coated with fibroblast growth factor-fibronectin (FGF-FN) fusion protein,” International Journal of Oral and Maxillofacial Implants, vol. 21, no. 6, pp. 859–866, 2006.
  71. S-H. Sohn, H.-K. Jun, and C.-S. Kim, et al., “Biological responses in osteoblast-like cell line according to thin layer hydroxyapatite coatings on anodized titanium,” Journal of Oral Rehabilitation, vol. 33, no. 12, pp. 898–911, 2006. View at Publisher · View at Google Scholar · View at PubMed
  72. M. Rouahi, O. Gallet, E. Champion, J. Dentzer, P. Hardouin, and K. Anselme, “Influence of hydroxyapatite microstructure on human bone cell response,” Journal of Biomedical Materials Research Part A, vol. 78, no. 2, pp. 222–235, 2006. View at Publisher · View at Google Scholar · View at PubMed
  73. A. L. Chun, J. G. Moralez, T. J. Webster, and H. Fenniri, “Helical rosette nanotubes: a biomimetic coating for orthopedics?,” Biomaterials, vol. 26, no. 35, pp. 7304–7309, 2005. View at Publisher · View at Google Scholar · View at PubMed
  74. H. Fenniri, P. Mathivanan, and K. L. Vidale, et al., “Helical rosette nanotubes: design, self-assembly, and characterization,” Journal of the American Chemical Society, vol. 123, no. 16, pp. 3854–3855, 2001. View at Publisher · View at Google Scholar
  75. G. K. Toworfe, R. J. Composto, C. S. Adams, I. M. Shapiro, and P. Ducheyne, “Fibronectin adsorption on surface-activated poly(dimethylsiloxane) and its effect on cellular function,” Journal of Biomedical Materials Research Part A, vol. 71, no. 3, pp. 449–461, 2004. View at Publisher · View at Google Scholar · View at PubMed
  76. A. F. von Recum and T. G. van Kooten, “The influence of micro-topography on cellular response and the implications for silicone implants,” Journal of Biomaterials Science, vol. 7, no. 2, pp. 181–198, 1995.
  77. Y. Yang, R. Cavin, and J. L. Ong, “Protein adsorption on titanium surfaces and their effect on osteoblast attachment,” Journal of Biomedical Materials Research Part A, vol. 67, no. 1, pp. 344–349, 2003. View at Publisher · View at Google Scholar · View at PubMed
  78. S. Bierbaum, R. Beutner, T. Hanke, D. Scharnweber, U. Hempel, and H. Worch, “Modification of Ti6Al4V surfaces using collagen I, III, and fibronectin—I: biochemical and morphological characteristics of the adsorbed matrix,” Journal of Biomedical Materials Research Part A, vol. 67, no. 2, pp. 421–430, 2003. View at Publisher · View at Google Scholar · View at PubMed
  79. A. S. Goldstein and P. A. DiMilla, “Examination of membrane rupture as a mechanism for mammalian cell detachment from fibronectin-coated biomaterials,” Journal of Biomedical Materials Research Part A, vol. 67, no. 2, pp. 658–666, 2003. View at Publisher · View at Google Scholar · View at PubMed
  80. J. van den Dolder, G. N. Bancroft, V. I. Sikavitsas, P. H. M. Spauwen, A. G. Mikos, and J. A. Jansen, “Effect of fibronectin- and collagen I-coated titanium fiber mesh on proliferation and differentiation of osteogenic cells,” Tissue Engineering, vol. 9, no. 3, pp. 505–515, 2003. View at Publisher · View at Google Scholar · View at PubMed
  81. R. Cornell, “Cell-substrate adhesion during cell culture. An ultrastructural study,” Experimental Cell Research, vol. 58, no. 2-3, pp. 289–295, 1969. View at Publisher · View at Google Scholar
  82. A. S. G. Curtis and M. Varde, “Control of cell behavior: topological factors,” Journal of the National Cancer Institute, vol. 33, pp. 15–26, 1964.
  83. M. Abercrombie, J. E. Heaysman, and S. M. Pegrum, “The locomotion of fibroblasts in culture—IV: electron microscopy of the leading lamella,” Experimental Cell Research, vol. 67, no. 2, pp. 359–367, 1971. View at Publisher · View at Google Scholar
  84. C. S. Izzard and L. R. Lochner, “Cell to substrate contacts in living fibroblasts: an interference reflexion study with an evaluation of the technique,” Journal of Cell Science, vol. 21, no. 1, pp. 129–159, 1976.
  85. N. Q. Balaban, U. S. Schwartz, and D. Riveline, et al., “Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates,” Nature Cell Biology, vol. 3, no. 5, pp. 466–472, 2001. View at Publisher · View at Google Scholar · View at PubMed
  86. M. Bigerelle and K. Anselme, “Bootstrap analysis of the relation between initial adhesive events and long-term cellular functions of human osteoblasts cultured on biocompatible metallic substrates,” Acta Biomaterialia, vol. 1, no. 5, pp. 499–510, 2005. View at Publisher · View at Google Scholar · View at PubMed
  87. Y. Wan, Y. Wang, and Z. Liu, et al., “Adhesion and proliferation of OCT-1 osteoblast-like cells on micro- and nano-scale topography structured poly(L-lactide),” Biomaterials, vol. 26, no. 21, pp. 4453–4459, 2005. View at Publisher · View at Google Scholar · View at PubMed
  88. A. S. Badami, M. R. Kreke, M. S. Thompson, J. S. Riffle, and A. S. Goldstein, “Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly(lactic acid) substrates,” Biomaterials, vol. 27, no. 4, pp. 596–606, 2006. View at Publisher · View at Google Scholar · View at PubMed
  89. Y. Ikarashi, T. Tsuchiya, M. Kaniwa, and A. Nakamura, “Activation of osteoblast-like MC3T3-E1 cell responses by poly(lactide),” Biological and Pharmaceutical Bulletin, vol. 23, no. 12, pp. 1470–1476, 2000.
  90. K. Isama and T. Tsuchiya, “Enhancing effect of poly(L-lactide) on the differentiation of mouse osteoblast-like MC3T3-E1 cells,” Biomaterials, vol. 24, no. 19, pp. 3303–3309, 2003. View at Publisher · View at Google Scholar
  91. H. Liu, E. B. Slamovich, and T. J. Webster, “Increased osteoblast functions among nanophase titania/poly(lactide-co-glycolide) composites of the highest nanometer surface roughness,” Journal of Biomedical Materials Research Part A, vol. 78, no. 4, pp. 798–807, 2006. View at Publisher · View at Google Scholar · View at PubMed
  92. K. M. Woo, J.-H. Jun, and V. J. Chen, et al., “Nano-fibrous scaffolding promotes osteoblast differentiation and biomineralization,” Biomaterials, vol. 28, no. 2, pp. 335–343, 2007. View at Publisher · View at Google Scholar · View at PubMed
  93. T. J. Webster and T. A. Smith, “Increased osteoblast function on PLGA composites containing nanophase titania,” Journal of Biomedical Materials Research Part A, vol. 74, no. 4, pp. 677–686, 2005. View at Publisher · View at Google Scholar · View at PubMed
  94. A. Diener, B. Nebe, and F. Lüthen, et al., “Control of focal adhesion dynamics by material surface characteristics,” Biomaterials, vol. 26, no. 4, pp. 383–392, 2005. View at Publisher · View at Google Scholar · View at PubMed
  95. D. S. W. Benoit and K. S. Anseth, “The effect on osteoblast function of colocalized RGD and PHSRN epitopes on PEG surfaces,” Biomaterials, vol. 26, no. 25, pp. 5209–5220, 2005. View at Publisher · View at Google Scholar · View at PubMed
  96. H. A. Biebuyck and G. M. Whitesides, “Self-organization of organic liquids on patterned self-assembled monolayers of alkanethiolates on gold,” Langmuir, vol. 10, no. 8, pp. 2790–2793, 1994. View at Publisher · View at Google Scholar
  97. H. Gau, S. Herminghaus, P. Lenz, and R. Lipowsky, “Liquid morphologies on structured surfaces: from microchannels to microchips,” Science, vol. 283, no. 5398, pp. 46–49, 1999. View at Publisher · View at Google Scholar
  98. E. Kim, Y. Xia, and G. M. Whitesides, “Polymer microstructures formed by moulding in capillaries,” Nature, vol. 376, no. 6541, pp. 581–584, 1995. View at Publisher · View at Google Scholar
  99. M. Morra, C. Cassinelli, and G. Cascardo, et al., “Surface engineering of titanium by collagen immobilization. Surface characterization and in vitro and in vivo studies,” Biomaterials, vol. 24, no. 25, pp. 4639–4654, 2003. View at Publisher · View at Google Scholar
  100. M. Sarikaya, C. Tamerler, A. K.-Y. Jen, K. Schulten, and F. Baneyx, “Molecular biomimetics: nanotechnology through biology,” Nature Materials, vol. 2, no. 9, pp. 577–585, 2003. View at Publisher · View at Google Scholar · View at PubMed
  101. Y. Xia and G. M. Whitesides, “Extending microcontact printing as a microlithographic technique,” Langmuir, vol. 13, no. 7, pp. 2059–2067, 1997. View at Publisher · View at Google Scholar
  102. S. Zhang, D. M. Marini, W. Hwang, and S. Santoso, “Design of nanostructured biological materials through self-assembly of peptides and proteins,” Current Opinion in Chemical Biology, vol. 6, no. 6, pp. 865–871, 2002. View at Publisher · View at Google Scholar
  103. S. M. Cutler and A. J. García, “Engineering cell adhesive surfaces that direct integrin α5β1 binding using a recombinant fragment of fibronectin,” Biomaterials, vol. 24, no. 10, pp. 1759–1770, 2003. View at Publisher · View at Google Scholar
  104. M. Nagai, T. Hayakawa, and A. Fukatsu, et al., “In vitro study of collagen coating of titanium implants for initial cell attachment,” Dental Materials Journal, vol. 21, no. 3, pp. 250–260, 2002.
  105. H. Schliephake, D. Scharnweber, M. Dard, S. Rößler, A. Sewing, and C. Hüttmann, “Biological performance of biomimetic calcium phosphate coating of titanium implants in the dog mandible,” Journal of Biomedical Materials Research Part A, vol. 64, no. 2, pp. 225–234, 2003. View at Publisher · View at Google Scholar · View at PubMed
  106. H. Shi, W.-B. Tsai, M. D. Garrison, S. Ferrari, and B. D. Ratner, “Template-imprinted nanostructured surfaces for protein recognition,” Nature, vol. 398, no. 6728, pp. 593–597, 1999. View at Publisher · View at Google Scholar · View at PubMed
  107. E. H. J. Danen, S.-I. Aota, A. A. van Kraats, K. M. Yamada, D. J. Ruiter, and G. N. P. van Muijen, “Requirement for the synergy site for cell adhesion to fibronectin depends on the activation state of integrin α5β1,” Journal of Biological Chemistry, vol. 270, no. 37, pp. 21612–21618, 1995. View at Publisher · View at Google Scholar
  108. S. Huveneers, I. van den Bout, P. Sonneveld, A. Sancho, A. Sonnenberg, and E. H. J. Danen, “Integrin αvβ3 controls activity and oncogenic potential of primed c-Src,” Cancer Research, vol. 67, no. 6, pp. 2693–2700, 2007. View at Publisher · View at Google Scholar · View at PubMed
  109. B. G. Keselowsky, A. W. Bridges, and K. L. Burns, et al., “Role of plasma fibronectin in the foreign body response to biomaterials,” Biomaterials, vol. 28, no. 25, pp. 3626–3631, 2007. View at Publisher · View at Google Scholar · View at PubMed
  110. B. G. Keselowsky, D. M. Collard, and A. J. García, “Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion,” Journal of Biomedical Materials Research Part A, vol. 66, no. 2, pp. 247–259, 2003. View at Publisher · View at Google Scholar · View at PubMed
  111. B. G. Keselowsky, D. M. Collard, and A. J. García, “Surface chemistry modulates focal adhesion composition and signaling through changes in integrin binding,” Biomaterials, vol. 25, no. 28, pp. 5947–5954, 2004. View at Publisher · View at Google Scholar · View at PubMed
  112. B. G. Keselowsky, D. M. Collard, and A. J. García, “Integrin binding specificity regulates biomaterial surface chemistry effects on cell differentiation,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 17, pp. 5953–5957, 2005. View at Publisher · View at Google Scholar · View at PubMed
  113. B. G. Keselowsky, L. Wang, Z. Schwartz, A. J. García, and B. D. Boyan, “Integrin α5 controls osteoblastic proliferation and differentiation responses to titanium substrates presenting different roughness characteristics in a roughness independent manner,” Journal of Biomedical Materials Research Part A, vol. 80, no. 3, pp. 700–710, 2007. View at Publisher · View at Google Scholar · View at PubMed
  114. K. Anselme, “Osteoblast adhesion on biomaterials,” Biomaterials, vol. 21, no. 7, pp. 667–681, 2000. View at Publisher · View at Google Scholar
  115. M. Kononen, M. Hormia, J. Kivilahti, J. Hautaniemi, and I. Thesleff, “Effect of surface processing on the attachment, orientation, and proliferation of human gingival fibroblasts on titanium,” Journal of Biomedical Materials Research, vol. 26, no. 10, pp. 1325–1341, 1992. View at Publisher · View at Google Scholar · View at PubMed
  116. D. Li, B. Liu, J. Wu, and J. Chen, “Bone interface of dental implants cytologically influenced by a modified sandblasted surface: a preliminary in vitro study,” Implant Dentistry, vol. 10, no. 2, pp. 132–138, 2001.
  117. A. S. G. Curtis and C. Wilkinson, “Nantotechniques and approaches in biotechnology,” Trends in Biotechnology, vol. 19, no. 3, pp. 97–101, 2001. View at Publisher · View at Google Scholar
  118. M. J. Dalby, L. Di Silvio, G. W. Davies, and W. Bonfield, “Surface topography and HA filler volume effect on primary human osteoblasts in vitro,” Journal of Materials Science: Materials in Medicine, vol. 11, no. 12, pp. 805–810, 2000. View at Publisher · View at Google Scholar
  119. J.-H. Wang, C.-H. Yao, W.-Y. Chuang, and T.-H. Young, “Development of biodegradable polyesterurethane membranes with different surface morphologies for the culture of osteoblasts,” Journal of Biomedical Materials Research, vol. 51, no. 4, pp. 761–770, 2000. View at Publisher · View at Google Scholar
  120. J. Domke, S. Dannöhl, W. J. Parak, O. Müller, W. K. Aicher, and M. Radmacher, “Substrate dependent differences in morphology and elasticity of living osteoblasts investigated by atomic force microscopy,” Colloids and Surfaces B, vol. 19, no. 4, pp. 367–379, 2000. View at Publisher · View at Google Scholar
  121. M. Gleiche, L. F. Chi, and H. Fuchs, “Nanoscopic channel lattices with controlled anisotropic wetting,” Nature, vol. 403, no. 6766, pp. 173–175, 2000. View at Publisher · View at Google Scholar · View at PubMed
  122. S. Lenhert, M.-B. Meier, U. Meyer, L. Chi, and H. P. Wiesmann, “Osteoblast alignment, elongation and migration on grooved polystyrene surfaces patterned by Langmuir-Blodgett lithography,” Biomaterials, vol. 26, no. 5, pp. 563–570, 2005. View at Publisher · View at Google Scholar · View at PubMed
  123. E. Martines, K. McGhee, C. Wilkinson, and A. S. G. Curtis, “A parallel-plate flow chamber to study initial cell adhesion on a nano featured surface,” IEEE Transactions on Nanobioscience, vol. 3, no. 2, pp. 90–95, 2004. View at Publisher · View at Google Scholar
  124. K. Mustafa, A. Wennerberg, J. Wroblewski, K. Hultenby, B. S. Lopez, and K. Arvidson, “Determining optimal surface roughness of TiO2 blasted titanium implant material for attachment, proliferation and differentiation of cells derived from human mandibular alveolar bone,” Clinical Oral Implants Research, vol. 12, no. 5, pp. 515–525, 2001. View at Publisher · View at Google Scholar
  125. K. Mustafa, J. Wroblewski, K. Hultenby, B. S. Lopez, and K. Arvidson, “Effects of titanium surfaces blasted with TiO2 particles on the initial attachment of cells derived from human mandibular bone: a scanning electron microscopic and histomorphometric analysis,” Clinical Oral Implants Research, vol. 11, no. 2, pp. 116–128, 2000. View at Publisher · View at Google Scholar
  126. K. Anselme, M. Bigerelle, and B. Noel, et al., “Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses,” Journal of Biomedical Materials Research, vol. 49, no. 2, pp. 155–166, 1999. View at Publisher · View at Google Scholar
  127. E. Eisenbarth, D. Velten, M. Müller, R. Thull, and J. Breme, “Nanostructured niobium oxide coatings influence osteoblast adhesion,” Journal of Biomedical Materials Research Part A, vol. 79, no. 1, pp. 166–175, 2006. View at Publisher · View at Google Scholar · View at PubMed
  128. H.-W. Kim, H.-E. Kim, and J. C. Knowles, “Fluor-hydroxyapatite sol-gel coating on titanium substrate for hard tissue implants,” Biomaterials, vol. 25, no. 17, pp. 3351–3358, 2004. View at Publisher · View at Google Scholar · View at PubMed
  129. S. Oh, C. Daraio, L.-H. Chen, T. R. Pisanic, R. R. Fiñones, and S. Jin, “Significantly accelerated osteoblast cell growth on aligned TiO2 nanotubes,” Journal of Biomedical Materials Research Part A, vol. 78, no. 1, pp. 97–103, 2006. View at Publisher · View at Google Scholar · View at PubMed
  130. A. A. Veis, S. Papadimitriou, P. Trisi, A. T. Tsirlis, N. A. Parissis, and J. N. Kenealy, “Osseointegration of Osseotite® and machined-surfaced titanium implants in membrane-covered critical-sized defects: a histologic and histometric study in dogs,” Clinical Oral Implants Research, vol. 18, no. 2, pp. 153–160, 2007. View at Publisher · View at Google Scholar · View at PubMed
  131. L. G. Persson, J. Mouhyi, T. Berglundh, L. Sennerby, and J. Lindhe, “Carbon dioxide laser and hydrogen peroxide conditioning in the treatment of periimplantitis: an experimental study in the dog,” Clinical Implant Dentistry and Related Research, vol. 6, no. 4, pp. 230–238, 2004. View at Publisher · View at Google Scholar
  132. L. Zhu, X. Ye, and G. Tang, et al., “Biomimetic coating of compound titania and hydroxyapatite on titanium,” Journal of Biomedical Materials Research Part A, 2007. View at Publisher · View at Google Scholar · View at PubMed
  133. L. Mancini, R. Tamma, and M. P. Settanni, et al., “Osteoblasts cultured on three-dimensional synthetic hydroxyapatite implanted on a chick Allantochorial membrane induce ectopic bone marrow differentiation,” Annals of the New York Academy of Sciences, 2007.
  134. C. Y. Yang, T. M. Lee, C. W. Yang, L. R. Chen, M. C. Wu, and T. S. Lui, “In vitro and in vivo biological responses of plasma-sprayed hydroxyapatite coatings with posthydrothermal treatment,” Journal of Biomedical Materials Research Part A, 2007. View at Publisher · View at Google Scholar · View at PubMed
  135. A. S. G. Curtis and C. Wilkinson, “New depths in cell behaviour: reactions of cells to nanotopography,” Biochemical Society Symposium, vol. 65, pp. 15–26, 1999.
  136. M. J. Dalby, S. J. Yarwood, M. O. Riehle, H. J. H. Johnstone, S. Affrossman, and A. S. G. Curtis, “Increasing fibroblast response to materials using nanotopography: morphological and genetic measurements of cell response to 13-nm-high polymer demixed islands,” Experimental Cell Research, vol. 276, no. 1, pp. 1–9, 2002. View at Publisher · View at Google Scholar · View at PubMed
  137. C. A. Scotchford, M. Ball, and M. Winkelmann, et al., “Chemically patterned, metal-oxide-based surfaces produced by photolithographic techniques for studying protein- and cell-interactions—II: protein adsorption and early cell interactions,” Biomaterials, vol. 24, no. 7, pp. 1147–1158, 2003. View at Publisher · View at Google Scholar
  138. J. M. Rice, J. A. Hunt, J. A. Gallagher, P. Hanarp, D. S. Sutherland, and J. Gold, “Quantitative assessment of the response of primary derived human osteoblasts and macrophages to a range of nanotopography surfaces in a single culture model in vitro,” Biomaterials, vol. 24, no. 26, pp. 4799–4818, 2003. View at Publisher · View at Google Scholar
  139. M. Rouahi, E. Champion, P. Hardouin, and K. Anselme, “Quantitative kinetic analysis of gene expression during human osteoblastic adhesion on orthopaedic materials,” Biomaterials, vol. 27, no. 14, pp. 2829–2844, 2006. View at Publisher · View at Google Scholar · View at PubMed
  140. C. Hendrich, U. Nöth, and U. Stahl, et al., “Testing of skeletal implant surfaces with human fetal osteoblasts,” Clinical Orthopaedics & Related Research, no. 394, pp. 278–289, 2002. View at Publisher · View at Google Scholar
  141. C. Hallgren, H. Reimers, J. Gold, and A. Wennerberg, “The importance of surface texture for bone integration of screw shaped implants: an in vivo study of implants patterned by photolithography,” Journal of Biomedical Materials Research, vol. 57, no. 4, pp. 485–496, 2001. View at Publisher · View at Google Scholar
  142. D. W. Hamilton, K. S. Wong, and D. M. Brunette, “Microfabricated discontinuous-edge surface topographies influence osteoblast adhesion, migration, cytoskeletal organization, and proliferation and enhance matrix and mineral deposition in vitro,” Calcified Tissue International, vol. 78, no. 5, pp. 314–325, 2006. View at Publisher · View at Google Scholar · View at PubMed
  143. Y. Germanier, S. Tosatti, N. Broggini, M. Textor, and D. Buser, “Enhanced bone apposition around biofunctionalized sandblasted and acid-etched titanium implant surfaces: a histomorphometric study in miniature pigs,” Clinical Oral Implants Research, vol. 17, no. 3, pp. 251–257, 2006. View at Publisher · View at Google Scholar · View at PubMed
  144. S. Rammelt, T. Illert, S. Bierbaum, D. Scharnweber, H. Zwipp, and W. Schneiders, “Coating of titanium implants with collagen, RGD peptide and chondroitin sulfate,” Biomaterials, vol. 27, no. 32, pp. 5561–5571, 2006. View at Publisher · View at Google Scholar · View at PubMed
  145. E. Slaets, G. Carmeliet, I. Naert, and J. Duyck, “Early cellular responses in cortical bone healing around unloaded titanium implants: an animal study,” Journal of Periodontology, vol. 77, no. 6, pp. 1015–1024, 2006. View at Publisher · View at Google Scholar · View at PubMed
  146. G. Vallés, P. González-Melendi, and J. L. González-Carrasco, et al., “Differential inflammatory macrophage response to rutile and titanium particles,” Biomaterials, vol. 27, no. 30, pp. 5199–5211, 2006. View at Publisher · View at Google Scholar · View at PubMed
  147. U. Müller, T. Imwinkelried, M. Horst, M. Sievers, and U. Graf-Hausner, “Do human osteoblasts grow into open-porous titanium?,” European Cells and Materials, vol. 11, pp. 8–15, 2006.
  148. J. P. Li, S. H. Li, C. A. Van Blitterswijk, and K. de Groot, “A novel porous Ti6A14V: characterization and cell attachment,” Journal of Biomedical Materials Research Part A, vol. 73, no. 2, pp. 223–233, 2005. View at Publisher · View at Google Scholar · View at PubMed
  149. B. C. Ward and T. Webster, “Effect of metal substrate nanometer topography on osteoblast metabolic activities,” in Biological and Bioinspired Materials and Devices, vol. 823, pp. 249–254, Materials Research Society, San Francisco, Calif, USA, April 2004.
  150. B. C. Ward and T. Webster, “Effect of metal substrate nanometer topography on osteoblast metabolic activities,” in TMS Conference Proceedings, vol. 3, pp. 13–17, 2005.
  151. J. K. Bibby, N. L. Bubb, D. J. Wood, and P. M. Mummery, “Fluorapatite-mullite glass sputter coated Ti6Al4V for biomedical applications,” Journal of Materials Science, vol. 16, no. 5, pp. 379–385, 2005. View at Publisher · View at Google Scholar · View at PubMed
  152. Z. M. Isa, G. B. Schneider, R. Zaharias, D. Seabold, and C. M. Stanford, “Effects of fluoride-modified titanium surfaces on osteoblast proliferation and gene expression,” The International Journal of Oral & Maxillofacial Implants, vol. 21, no. 2, pp. 203–211, 2006.
  153. S. Ber, G. Torun Köse, and V. Hasirci, “Bone tissue engineering on patterned collagen films: an in vitro study,” Biomaterials, vol. 26, no. 14, pp. 1977–1986, 2005. View at Publisher · View at Google Scholar · View at PubMed
  154. A. J. García and C. D. Reyes, “Bio-adhesive surfaces to promote osteoblast differentiation and bone formation,” Journal of Dental Research, vol. 84, no. 5, pp. 407–413, 2005.
  155. M. Jäger, T. Feser, H. Denck, and R. Krauspe, “Proliferation and osteogenic differentiation of mesenchymal stem cells cultured onto three different polymers in vitro,” Annals of Biomedical Engineering, vol. 33, no. 10, pp. 1319–1332, 2005. View at Publisher · View at Google Scholar · View at PubMed
  156. Y. Yonggang, J. G. Wolke, L. Yubao, and J. A. Jansen, “In vitro evaluation of different heat-treated radio frequency magnetron sputtered calcium phosphate coatings,” Clinical Oral Implants Research, vol. 18, no. 3, pp. 345–353, 2007. View at Publisher · View at Google Scholar · View at PubMed
  157. B. Baumann, C. P. Rader, and J. Seufert, et al., “Effects of polyethylene and TiAIV wear particles on expression of RANK, RANKL and OPG mRNA,” Acta Orthopaedica Scandinavica, vol. 75, no. 3, pp. 295–302, 2004.
  158. J. Huang, S. M. Best, and W. Bonfield, et al., “In vitro assessment of the biological response to nano-sized hydroxyapatite,” Journal of Materials Science, vol. 15, no. 4, pp. 441–445, 2004. View at Publisher · View at Google Scholar
  159. K. Peters, R. E. Unger, C. J. Kirkpatrick, A. M. Gatti, and E. Monari, “Effects of nano-scaled particles on endothelial cell function in vitro: studies on viability, proliferation and inflammation,” Journal of Materials Science, vol. 15, no. 4, pp. 321–325, 2004. View at Publisher · View at Google Scholar
  160. S. Bruni, M. Martinesi, M. Stio, C. Treves, T. Bacci, and F. Borgioli, “Effects of surface treatment of Ti-6AI-4V titanium alloy on biocompatibility in cultured human umbilical vein endothelial cells,” Acta Biomaterialia, vol. 1, no. 2, pp. 223–234, 2005. View at Publisher · View at Google Scholar · View at PubMed
  161. A. S. Eriksson and P. Thomsen, “Leukotriene B4, interleukin 1 and leucocyte accumulation in titanium and PTFE chambers after implantation in the rat abdominal wall,” Biomaterials, vol. 12, no. 9, pp. 827–830, 1991. View at Publisher · View at Google Scholar
  162. F. Giudiceandrea, A. Iacona, and G. Cervelli, et al., “Mechanisms of bone resorption: analysis of proinflammatory cytokines in peritoneal macrophages from titanium implant—an experimental design,” Journal of Craniofacial Surgery, vol. 9, no. 3, pp. 254–259, 1998. View at Publisher · View at Google Scholar
  163. M. Martinesi, S. Bruni, M. Stio, C. Treves, and F. Borgioli, “In vitro interaction between surface-treated Ti-6Al-4V titanium alloy and human peripheral blood mononuclear cells,” Journal of Biomedical Materials Research Part A, vol. 74, no. 2, pp. 197–207, 2005. View at Publisher · View at Google Scholar · View at PubMed
  164. A. K. Refai, M. Textor, D. M. Brunette, and J. D. Waterfield, “Effect of titanium surface topography on macrophage activation and secretion of proinflammatory cytokines and chemokines,” Journal of Biomedical Materials Research Part A, vol. 70, no. 2, pp. 194–205, 2004. View at Publisher · View at Google Scholar · View at PubMed
  165. W. G. Brodbeck, J. Patel, and G. Voskerician, et al., “Biomaterial adherent macrophage apoptosis is increased by hydrophilic and anionic substrates in vivo,” Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 16, pp. 10287–10292, 2002. View at Publisher · View at Google Scholar · View at PubMed
  166. S. Morais, N. Dias, J. P. Sousa, M. H. Fernandes, and G. S. Carvalho, “In vitro osteoblastic differentiation of human bone marrow cells in the presence of metal ions,” Journal of Biomedical Materials Research, vol. 44, no. 2, pp. 176–190, 1999. View at Publisher · View at Google Scholar
  167. S. Morais, J. P. Sousa, M. H. Fernandes, G. S. Carvalho, J. D. de Bruijn, and C. A. van Blitterswijk, “Effects of AISI 316L corrosion products in in vitro bone formation,” Biomaterials, vol. 19, no. 11-12, pp. 999–1007, 1998. View at Publisher · View at Google Scholar
  168. D. A. Puleo and W. W. Huh, “Acute toxicity of metal ions in cultures of osteogenic cells derived from bone marrow stromal cells,” Journal of Applied Biomaterials, vol. 6, no. 2, pp. 109–116, 1995. View at Publisher · View at Google Scholar · View at PubMed
  169. H.-Y. Lin and J. D. Bumgardner, “Changes in the surface oxide composition of Co-Cr-Mo implant alloy by macrophage cells and their released reactive chemical species,” Biomaterials, vol. 25, no. 7-8, pp. 1233–1238, 2004. View at Publisher · View at Google Scholar
  170. H.-Y. Lin and J. D. Bumgardner, “In vitro biocorrosion of Ti-6AI-4V implant alloy by a mouse macrophage cell line,” Journal of Biomedical Materials Research Part A, vol. 68, no. 4, pp. 717–724, 2004. View at Publisher · View at Google Scholar · View at PubMed
  171. H.-Y. Lin and J. D. Bumgardner, “In vitro biocorrosion of Co-Cr-Mo implant alloy by macrophage cells,” Journal of Orthopaedic Research, vol. 22, no. 6, pp. 1231–1236, 2004. View at Publisher · View at Google Scholar · View at PubMed
  172. A. Hunter, C. W. Archer, P. S. Walker, and G. W. Blunn, “Attachment and proliferation of osteoblasts and fibroblasts on biomaterials for orthopaedic use,” Biomaterials, vol. 16, no. 4, pp. 287–295, 1995. View at Publisher · View at Google Scholar
  173. S. Kuroda, S. Takeda, and M. Nakamura, “Effects of six particulate metals on osteoblast-like MG-63 and HOS cells in vitro,” Dental Materials Journal, vol. 22, no. 4, pp. 507–520, 2003.
  174. D. Granchi, I. Amato, and L. Battistelli, et al., “Molecular basis of osteoclastogenesis induced by osteoblasts exposed to wear particles,” Biomaterials, vol. 26, no. 15, pp. 2371–2379, 2005. View at Publisher · View at Google Scholar · View at PubMed
  175. C. P. Rader, B. Baumann, and O. Rolf, et al., “Detection of differentially expressed genes in particle disease using array-filter analysis,” Biomedizinische Technik, vol. 47, no. 5, pp. 111–116, 2002.
  176. D. T. O'Connor, M. G. Choi, S. Y. Kwon, and K.-L. Paul Sung, “New insight into the mechanism of hip prosthesis loosening: effect of titanium debris size on osteoblast function,” Journal of Orthopaedic Research, vol. 22, no. 2, pp. 229–236, 2004. View at Publisher · View at Google Scholar · View at PubMed
  177. K. Miyanishi, M. C. Trindade, T. Ma, S. B. Goodman, D. J. Schurman, and R. L. Smith, “Periprosthetic osteolysis: induction of vascular endothelial growth factor from human monocyte/macrophages by orthopaedic biomaterial particles,” Journal of Bone and Mineral Research, vol. 18, no. 9, pp. 1573–1583, 2003.
  178. Y. Nakashima, D.-H. Sun, and M. C. Trindade, et al., “Signaling pathways for tumor necrosis factor-α and interleukin-6 expression in human macrophages exposed to titanium-alloy particulate debris in vitro,” Journal of Bone and Joint Surgery, American, vol. 81, no. 5, pp. 603–615, 1999.
  179. D. D. Dean, Z. Schwartz, and Y. Liu, et al., “The effect of ultra-high molecular weight polyethylene wear debris on MG-63 osteosarcoma cells in vitro,” Journal of Bone and Joint Surgery, American, vol. 81, no. 4, pp. 452–461, 1999.
  180. E. Ingham and J. Fisher, “The role of macrophages in osteolysis of total joint replacement,” Biomaterials, vol. 26, no. 11, pp. 1271–1286, 2005. View at Publisher · View at Google Scholar · View at PubMed
  181. T. R. Green, J. Fisher, M. Stone, B. M. Wroblewski, and E. Ingham, “Polyethylene particles of a ‘critical size’ are necessary for the induction of cytokines by macrophages in vitro,” Biomaterials, vol. 19, no. 24, pp. 2297–2302, 1998. View at Publisher · View at Google Scholar
  182. J. Yao, T. T. Glant, and M. W. Lark, et al., “The potential role of fibroblasts in periprosthetic osteolysis: fibroblast response to titanium particles,” Journal of Bone and Mineral Research, vol. 10, no. 9, pp. 1417–1427, 1995.
  183. M. Manlapaz, W. J. Maloney, and R. L. Smith, “In vitro activation of human fibroblasts by retrieved titanium alloy wear debris,” Journal of Orthopaedic Research, vol. 14, no. 3, pp. 465–472, 1996. View at Publisher · View at Google Scholar · View at PubMed
  184. A. S. Shanbhag, J. J. Jacobs, J. Black, J. O. Galante, and T. T. Giant, “Effects of particles on fibroblast proliferation and bone resorption in vitro,” Clinical Orthopaedics & Related Research, no. 342, pp. 205–217, 1997.
  185. S. Santavirta, M. Takagi, L. Nordsletten, A. Anttila, R. Lappalainen, and Y. T. Konttinen, “Biocompatibility of silicon carbide in colony formation test in vitro: a promising new ceramic THR implant coating material,” Archives of Orthopaedic and Trauma Surgery, vol. 118, no. 1-2, pp. 89–91, 1998. View at Publisher · View at Google Scholar
  186. J.-S. Sun, F.-H. Lin, T.-Y. Hung, Y.-H. Tsuang, W. H.-S. Chang, and H.-C. Liu, “The influence of hydroxyapatite particles on osteoclast cell activities,” Journal of Biomedical Materials Research, vol. 45, no. 4, pp. 311–321, 1999. View at Publisher · View at Google Scholar
  187. T. R. Green, J. Fisher, J. B. Matthews, M. H. Stone, and E. Ingham, “Effect of size and dose on bone resorption activity of macrophages by in vitro clinically relevant ultra high molecular weight polyethylene particles,” Journal of Biomedical Materials Research, vol. 53, no. 5, pp. 490–497, 2000. View at Publisher · View at Google Scholar
  188. T. Akisue, T. W. Bauer, C. F. Farver, and Y. Mochida, “The effect of particle wear debris on NFκB activation and pro-inflammatory cytokine release in differentiated THP-1 cells,” Journal of Biomedical Materials Research, vol. 59, no. 3, pp. 507–515, 2002. View at Publisher · View at Google Scholar · View at PubMed
  189. A. Wilke, S. Endres, P. Griss, and U. Herz, “Cytokine profile of a human bone marrow cell culture on exposure to titanium-aluminium-vanadium particles,” Zeitschrift für Orthopädie und Ihre Grenzgebiete, vol. 140, no. 1, pp. 83–89, 2002. View at Publisher · View at Google Scholar · View at PubMed
  190. M. A. Germain, A. Hatton, and S. Williams, et al., “Comparison of the cytotoxicity of clinically relevant cobalt-chromium and alumina ceramic wear particles in vitro,” Biomaterials, vol. 24, no. 3, pp. 469–479, 2003. View at Publisher · View at Google Scholar
  191. G. I. Howling, H. Sakoda, and A. Antonarulrajah, et al., “Biological response to wear debris generated in carbon based composites as potential bearing surfaces for artificial hip joints,” Journal of Biomedical Materials Research Part B, vol. 67, no. 2, pp. 758–764, 2003. View at Publisher · View at Google Scholar · View at PubMed
  192. D. Granchi, G. Ciapetti, and I. Amato, et al., “The influence of alumina and ultra-high molecular weight polyethylene particles on osteoblast-osteoclast cooperation,” Biomaterials, vol. 25, no. 18, pp. 4037–4045, 2004. View at Publisher · View at Google Scholar · View at PubMed
  193. G. I. Howling, E. Ingham, and H. Sakoda, et al., “Carbon-carbon composite bearing materials in hip arthroplasty: analysis of wear and biological response to wear debris,” Journal of Materials Science, vol. 15, no. 1, pp. 91–98, 2004. View at Publisher · View at Google Scholar
  194. C. C. Barrias, C. C. Ribeiro, M. Lamghari, C. S. Miranda, and M. A. Barbosa, “Proliferation, activity, and osteogenic differentiation of bone marrow stromal cells cultured on calcium titanium phosphate microspheres,” Journal of Biomedical Materials Research Part A, vol. 72, no. 1, pp. 57–66, 2005. View at Publisher · View at Google Scholar · View at PubMed
  195. A. Petit, F. Mwale, J. Antoniou, D. J. Zukor, and O. L. Huk, “Effect of bisphosphonates on the stimulation of macrophages by alumina ceramic particles: a comparison with ultra-high-molecular-weight polyethylene,” Journal of Materials Science, vol. 17, no. 7, pp. 667–673, 2006. View at Publisher · View at Google Scholar · View at PubMed
  196. W. B. Tan, N. Huang, and Y. Zhang, “Ultrafine biocompatible chitosan nanoparticles encapsulating multi-coloured quantum dots for bioapplications,” Journal of Colloid and Interface Science, vol. 310, no. 2, pp. 464–470, 2007. View at Publisher · View at Google Scholar · View at PubMed
  197. C. M. Sayes, F. Liang, and J. L. Hudson, et al., “Functionalization density dependence of single-walled carbon nanotubes cytotoxicity in vitro,” Toxicology Letters, vol. 161, no. 2, pp. 135–142, 2006. View at Publisher · View at Google Scholar · View at PubMed
  198. S. Koyama, H. Haniu, and K. Osaka, et al., “Medical application of carbon-nanotube-filled nanocomposites: the microcatheter,” Small, vol. 2, no. 12, pp. 1406–1411, 2006. View at Publisher · View at Google Scholar · View at PubMed
  199. A. V. Liopo, M. P. Stewart, J. Hudson, J. M. Tour, and T. C. Pappas, “Biocompatibility of native and functionalized single-walled carbon nanotubes for neuronal interface,” Journal of Nanoscience and Nanotechnology, vol. 6, no. 5, pp. 1365–1374, 2006. View at Publisher · View at Google Scholar
  200. M. Nahar, T. Dutta, and S. Murugesan, et al., “Functional polymeric nanoparticles: an efficient and promising tool for active delivery of bioactives,” Critical Reviews in Therapeutic Drug Carrier Systems, vol. 23, no. 4, pp. 259–318, 2006.
  201. H. Warashina, S. Sakano, and S. Kitamura, et al., “Biological reaction to alumina, zirconia, titanium and polyethylene particles implanted onto murine calvaria,” Biomaterials, vol. 24, no. 21, pp. 3655–3661, 2003. View at Publisher · View at Google Scholar