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Journal of Biomedicine and Biotechnology
Volume 2007 (2007), Article ID 76514, 9 pages
http://dx.doi.org/10.1155/2007/76514
Research Article

Imaging GABAc Receptors with Ligand-Conjugated Quantum Dots

1Department of Chemistry, Vanderbilt University, Station B 311822, Nashville, TN 37235, USA
2Lions of Illinois Eye Research Institute, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL 60612, USA
3Department of Neurology and Rehabilitation, University of Illinois at Chicago, Chicago, IL 60612, USA

Received 14 May 2007; Revised 30 August 2007; Accepted 21 December 2007

Academic Editor: Marek Osinski

Copyright © 2007 Ian D. Tomlinson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We report a methodology for labeling the GABAC receptor on the surface membrane of intact cells. This work builds upon our earlier work with serotonin-conjugated quantum dots and our studies with PEGylated quantum dots to reduce nonspecific binding. In the current approach, a PEGylated derivative of muscimol was synthesized and attached via an amide linkage to quantum dots coated in an amphiphilic polymer derivative of a modified polyacrylamide. These conjugates were used to image GABAC receptors heterologously expressed in Xenopus laevis oocytes.