Fluorescent Nanoparticle-Based Indirect Immunofluorescence Microscopy for Detection 
      of <i>Mycobacterium tuberculosis</i>

Qin, Dilan; He, Xiaoxiao; Wang, Kemin; Zhao, Xiaojun Julia; Tan, Weihong; Chen, Jiyun

doi:https://doi.org/10.1155/2007/89364

BioMed Research International

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Biomedical Applications of Colloidal Nanocrystals

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Volume 2007 | Article ID 089364 | https://doi.org/10.1155/2007/89364

Fluorescent Nanoparticle-Based Indirect Immunofluorescence Microscopy for Detection of Mycobacterium tuberculosis

Dilan Qin,^1,2Xiaoxiao He,^1,2Kemin Wang,^1,2Xiaojun Julia Zhao,³Weihong Tan,^1,2and Jiyun Chen^1,2

Academic Editor: Marek Osinski

Received24 Mar 2007

Revised10 Jul 2007

Accepted10 Oct 2007

Published25 Nov 2007

Abstract

A method of fluorescent nanoparticle-based indirect immunofluorescence microscopy (FNP-IIFM) was developed for the rapid detection of Mycobacterium tuberculosis. An anti-Mycobacterium tuberculosis antibody was used as primary antibody to recognize Mycobacterium tuberculosis, and then an antibody binding protein (Protein A) labeled with Tris(2,2-bipyridyl)dichlororuthenium(II) hexahydrate (RuBpy)-doped silica nanoparticles was used to generate fluorescent signal for microscopic examination. Prior to the detection, Protein A was immobilized on RuBpy-doped silica nanoparticles with a coverage of ∼5.1×102 molecules/nanoparticle. With this method, Mycobacterium tuberculosis in bacterial mixture as well as in spiked sputum was detected. The use of the fluorescent nanoparticles reveals amplified signal intensity and higher photostability than the direct use of conventional fluorescent dye as label. Our preliminary studies have demonstrated the potential application of the FNP-IIFM method for rapid detection of Mycobacterium tuberculosis in clinical samples.

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Copyright © 2007 Dilan Qin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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