Differential Effects of Leptin on the Invasive Potential of Androgen-Dependent and -Independent Prostate Carcinoma Cells
Figure 3
Leptin stimulates MAPK
phosphorylation in PC-3 and DU-145 cells. Growth factor-starved PC-3 (a) or DU-145 cells (c) were exposed to increasing
concentration of leptin for various time points. Cell lysates obtained after
each time point were separated on a 7.5% SDS-PAGE and Western-blotted. Phosphorylated
MAPK levels were obtained after incubation with p44/42 MAPK antibody and
chemiluminescent detection. (b) and
(d), levels of p44/42 MAPK obtained after densitometric analysis and
represented as percentage of control against time (control being set at 100%).
The results of one of the three independent experiments are shown.
