Competition of Lactobacillus paracasei with Salmonella enterica for Adhesion to Caco-2 Cells

Figure 2

Two-hour coincubation experiments. Adhesion of S. enterica to Caco-2 cells under different conditions: (a) S. enterica was incubated with Caco-2 cells; (b) S. enterica and L. paracasei were coincubated; (c) L.paracasei overnight culture was washed with and resuspended in isotonic salt solution and coincubated with S. enterica; (d) L. paracasei overnight culture was centrifuged and spent supernatant (SCS) was incubated with S. enterica. S. enterica was incubated with SCS fractions: (e) I (>30 kDa); (f) II (30–10 kDa); (g) III (10–5 kDa); (h)IV (<5 kDa). Numbers represent the amount of adhered bacteria per 1 Caco-2 cell. Mean and SEM, t-test. Asterisks indicate the statistical differences: ^*𝑃<.05, ^**𝑃<.01, ^***𝑃<.001.