CFP and YFP, but Not GFP, Provide Stable Fluorescent Marking of Rat Hepatic Adult Stem Cells
Figure 1
Transient, short-term GFP fluorescence expression by the rat hepatic ASC
strain, Lig-8, is associated with cell death. Cell strain, Lig-8, was
transfected with the pEGFP-N3 expression vector. Shown are images of 5
independent colonies transiently expressing GFP fluorescence at 72–96 hours
posttransfection. Shown are the phases (a)–(e), GFP-fluorescence (f)–(j), propidium
iodide (PI) fluorescence (k)–(o), and merged GFP and PI fluorescence (p)–(t)
images. Arrows highlight specific examples of cells double-positive for GFP and
PI fluorescence in Colony no. 12. Scale bar is equivalent to 100 μm.