CFP and YFP, but Not GFP, Provide Stable Fluorescent Marking of Rat Hepatic Adult Stem Cells
Figure 5
CFP-fluorescence expression of the rat hepatic ASC clone B3 is stable after
induction of differentiation. Flow
cytometry analysis was performed with cultures of the nontransfected parental
rat hepatic ASC strain, Lig-8 (a), and CFP-fluorescent clone B3 hepatic ASC
cultures. The B3 cells were evaluated under routine (b) and differentiating (c)
culture conditions. Histograms plot the relative numbers of cells as a function
of the log-relative CFP fluorescence per cell. The background fluorescence, as
defined by nontransfected Lig-8 cells is depicted as the R1 region, and
positive fluorescence is denoted by the R2 region. Numbers indicate the percent
of cells positive for CFP fluorescence expression.
