CFP and YFP, but Not GFP, Provide Stable Fluorescent Marking of Rat Hepatic Adult Stem Cells

Table 1

Relative transfection efficiency of fluorescent gene markers and cloning efficiency of selected transfected cell colonies. Transfection efficiency is defined as the average number of colonies/estimated number of cells transfected/μg DNA. Transfections included ∼1.5×10⁶ cells. Cloning efficiency is defined as the number of stable cell strains derived/number of colonies cultured.

Fluorescent marker	Average colony number/10-cm dish	Relative transfection efficiency	Number of clones picked	Number of Stable cell strains derived	Cloning efficiency
GFP	1.3 (𝑛=10)	0.02	8	0	0%
CFP	64 (𝑛=3)	1.00	6	6	100%
YFP	61 (𝑛=3)	0.95	6	6	100%