A Sensitive and Rapid Assay for Investigating Vertical Transmission of Hepatitis B Virus via Male Germ Line Using EGFP Vector as Reporter

<table>RT-PCR product of HBs gene: M: Markers; 1, 2, and 3: the samples from three
single embryos with green fluorescence showing HBs cDNA positive bands, respectively; 4,
5, and 6: the samples from three single embryos without green fluorescence showing negative
results for HBs DNA; 7: the negative control (−RT); 8: the negative control (−T).</table>

BioMed Research International

fig6

Figure 6

Figure 6: A Sensitive and Rapid Assay for Investigating Vertical Transmission of Hepatitis B Virus via Male Germ Line Using EGFP Vector as Reporter 