Protein Alterations in Infiltrating Ductal Carcinomas of the Breast as Detected by Nonequilibrium pH Gradient Electrophoresis and Mass Spectrometry

Table 1

Results of MALDI-TOF mass spectra and database searching for protein identification.

Identified proteins	Spot^(a) number	Mr(kDa)/ pI^(b)	Access N^°(c)	Peptides matched	Relative^(d) volume (%)		Mass accuracy (ppm)	Sequence coverage %	Reported function
					N	T
Complement component C3c (chainD)	1	71317/6.82	78101272	36	0.085±0.015	0.02±0.007	45	64	Immune response
IGHG1 protein	2	45286/8.63	44890623	9	0.121±0.001	0.021±0.008	42	27	Immune response
IGHG1 protein	3	45286/8.63	44890623	9	0.142±0.008	0.041±0.006	43	29	Immune response
IGHG1 protein	4	45286/8.63	44890623	7	0.12±0.004	0.019±0.009	50	24	Immune response
IGHG1 protein	5	45286/8.63	44890623	7	0.101±0.006	0.014±0.005	50	24	Immune response
IGHG1 protein	6	45286/8.63	44890623	7	0.095±0.007	0.017±0.006	50	24	Immune response
Carbonic anhydrase 1	7	28909/6.59	4502517	13	0.081±0.01	0.018±0.009	72	72	metabolic enzyme
Enolase1	8	47481/7.01	4503571	30	0.012±0.001	0.131±0.006	41	62	Glycolytic enzyme
phosphoglycerate kinase 1	9	44973/8.30	48145549	28	0.015±0.007	0.108±0.03	60	58	Glycolytic enzyme
Deoxy hemoglobin (ChainD)	10	15928/7.14	27574253	11	0.018±0.004	0.123±0.006	49	79	O2/CO2 transport
Deoxy hemoglobin (ChainD)	11	15928/7.14	27574253	11	0.022±0.002	0.131±0.002	49	80	O2/CO2 transport
Manganese superoxide dismutase	12	22290/6.86	2780819	9	0.019±0.006	0.075±0.001	50	57	Anti-oxidant enzyme
Alpha B-Crystallin	13	20146/6.76	13937813	11	0.022±0.001	0.097±0.014	57	56	Chaperone
Raf Kinase inhibitor protein	14	16068/8,81	4261934	9	0.019±0.008	0.132±0.015	45	54	Signaltrans- duction
Peptidyl- prolylisomerase A	15	18228/7,68	13937981	13	0.02±0.007	0.109±0.001	67	59	Immuno- philin
Peptidyl- prolylisomerase A	16	18228/7,68	13937981	14	0.011±0.004	0.102±0.005	67	60	Immuno- philin
Cofilin1	17	18719/8.22	15147369	8	0.018±0.008	0.112±0.012	35	45	Actinturnover
HSP27	18	22427/7.83	662841	12	0.021±0.001	0.117±0.003	48	51	Chaperone
HSP27	19	22427/7.83	662841	16	0.014±0.008	0.097±0.005	48	67	Chaperone
hnRNPA2/B1 isoform A2	20	36041/8.67	4504447	21	0.015±0.007	0.141±0.021	35	53	Several functions

^(a) Index in the reference gel (Figure 1).
^(b) Expected Mr and pI which were calculated on the complete sequences.
^(c) Accession number in NCBI.
^(d) The individual spot volumes were expressed as a percentage of the total volume in all of the spots present in the gel. N: nontumor tissue; T: tumor tissue.