Review Article
Metabolic Control Analysis: A Tool for Designing Strategies to Manipulate Metabolic Pathways
Table 2
GSH and phytochelatin synthesis enzymes overexpression in plants and yeasts.
| Overexpressed enzyme (activity fold) | Organism (experimental condition) | Metabolite | Reference | (increment fold) |
| ATP sulfurylase (2.1) | Brassica juncea | 2.1 [GSH] | [37] | ATP sulfurylase (4.8) | Tobacco (unstressed) | 1.3 [S] | [38] | O-acetyl-serine thiol-lyase (2.5) | Tobacco (unstressed) | 2 [Cys] | [39] | 0 [GSH] | Serine acetyl transferase
(>10) | Potato chloroplasts (unstressed) | 2 [Cys] | [40] | 0 [GSH] | E. coli GS (90) | Populus tremula (unstressed) | 0 [GSH] | [34] | GS (3) | S. cerevisiae (unstressed) | 0 [GSH] | [41] | E. coli γ-ECS (>2) | Brassica juncea (unstressed) | 0 [GSH] | [35] | B. juncea
(+100 M C d2+) | 4 [GSH](a) | γ-ECS (2.1) | S. cerevisiae (unstressed)) | 1.3 [GSH] | [42] | E. coli γ-ECS (50) | Populus tremula (unstressed) | 4.6 [GSH] | [34] | E. coli γ-ECS (4.9) | Brassica juncea (unstressed) | 3.5 [GSH](b) | [43] | B. juncea (+200 M C d2+) | 1.5 [GSH](b) | E. coli γ-ECS (40) | Tobacco (unstressed) | >4 [GSH] | [44] | γ-ECS (9.1) + GS (18) | S. cerevisiae (unstressed) | 1.8 [GSH] | [45] | PCS (>2) | Arabidopsis thaliana (+85 M C d2+) | 0 [GSH] | [36] | Vacuolar transporter of PC-Cd complexes (>2) | S. pombe | Higher C | [46] | resistance |
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(a)The
increase was only in roots with no effect on shoots. (b)The increase was only in shoots with
no effect on roots.
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