The Cyclodextrin Glycosyltransferase of Paenibacillus pabuli US132 Strain: Molecular Characterization and Overproduction of the Recombinant Enzyme
Figure 2
Comparison of the deduced amino acid sequence of
US132 CGTase with those of related CGTases. The five domains found in CGTase
proteins are indicated by letters A to E under the sequences. The four highly
conserved regions are boxed and the catalytic residues are shaded. Arrows
indicate the main amino acid residues of cyclization activity Lys47, Tyr89,
Asn94, Phe183, Asn193, Leu194, Tyr195, Asp196, Phe259, Phe283, and Asp371. The
numbering starts after the respective signal sequence, with identity (*),
strongly similar (:), and weakly similar (). PP132: CGTase of Paenibacillus
pabuli US132 strain; BC8: CGTase of Bacillus circulans strain no. 8;
Bli: CGTase of Bacillus licheniformis; BC251: CGTase of Bacillus
circulans strain 251; Bsp1011: CGTase of Bacillus sp.1011.
