Proteome-Level Responses of Escherichia coli to Long-Chain Fatty Acids and Use of Fatty Acid Inducible Promoter in Protein Production

Table 1

Bacterial strains and plasmids used in this study.

Strain or plasmid	Relevant characteristics	Reference or source
E. coli strains
XL1-Blue	recA1, endA1, gyrA96, thi, hsdR17, suppE44, relA1, l^-, lac^-, F’[proAB lacl^q lacZ ΔM15, Tn10 (tet)^r]	Stratagene^a
W3110	F^-mcrA mcrB IN(rrnD^-rrnE)1𝜆^-	KCTC^b
Plasmids
pTac99A	pTrc99A derivative; tac promoter, cloning vehicle; Ap^r	Park and Lee [9]
pAD99A	pTac99A derivative; aldehyde dehydrogenase (aldA) promoter; Ap^r	This study
pUP99A	pTac99A derivative; uridine phosphorylase (udp) promoter; Ap^r	This study
pGFPuv	Ap^r, lac promoter, gfp	Clontech^c
pAD99GFP	pAD99A derivative; gfp	This study
pTac99GFP	pTac99A derivative; gfp	This study
pUP99GFP	pUP99A derivative; gfp	This study

^aStratagene Cloning System (La Jolla, Calif, USA).
^bKorean Collection for Type Cultures, (Daejeon, Korea).
^cBD Biosciences Clontech (Palo Alto, Calif, USA).