|
Spot no. | Protein name | Method for identity | Accession no. | pI/Mwa (kDa) | Protein description | Fold changeb |
O1/G1 | O2/G2 |
|
1 | AcnB | Gel match | P36683 | 5.24/75.9 | Aconitate hydratase 2 | | |
2 | AsnS | Gel match | P17242 | 5.64/92.8 | Asparaginyl-tRNA synthetase | — | — |
3 | AceF | Gel match | P06959 | 5.01/77.5 | Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex | | — |
4 | DnaK | Gel match | P04475 | 4.81/69.6 | Chaperone protein DnaK | | |
5 | PtsI | Gel match | P08839 | 4.78/59.8 | Phosphoenolpyruvate-protein
phosphotransferase | | 3 |
6 | AldA | MS/MS | P25553 | 5.07/52.2 | Aldehyde dehydrogenase A | Appeared | Appeared |
7 | MopA (GroEL) | Gel match | P06139 | 4.85/56.7 | 60 kDa chaperonin (GroEL protein) | | |
8 | Tig | Gel match | P22257 | 4.83/51.0 | Trigger factor (TF) | — | — |
9 | HtpG | Gel match | P10413 | 5.06/65.6 | Chaperone protein HtpG (Heat shock protein HtpG) | — | — |
10 | AtpD | Gel match | P00824 | 4.90/47.7 | ATP synthase beta chain | — | — |
11 | Icd (IcdA) | Gel match | P08200 | 5.02/46.0 | Isocitrate dehydrogenase | — | |
12 | AceA1 | MS/MS | P05313 | 5.19/44.1 5.12/44.1 | Isocitrate
lyase | 5 | 7 |
| AceA2 | MS/MS | | 5.01/33.8 | Isocitrate lyase fragment | 6 | 7 |
13 | GlnA | Gel
match | P06711 | 5.25/53.8 | Glutamine synthetase | — | — |
14 | IlvC | Gel match | P05793 | 5.26/52.0 | Ketol-acid reductoisomerase | — | — |
15 | GlpK | Gel
match | P08859 | 5.30/50.6 | Glycerol kinase (Glycerokinase) | — | — |
16 | Eno | Gel match | P08324 | 5.34/46.5 5.29/46.2 | Enolase
(2-phosphoglycerate dehydratase) | — | |
17 | TufA (EF-Tu) | Gel match | P02990 | 5.32/44.6 | Elongation factor Tu (EF-Tu) | — | — |
18 | FabD (TfpA) | Gel match | P25715 | 5.37/44.8 | Malonyl CoA-acyl carrier protein transacylase | | — |
19 | LeuC | Gel match | P30127 | 5.42/44.4 5.95/51.6 | 3-isopropylmalate
dehydratase large subunit | | — |
20 | FadB | MS/MS | P21177 | 5.84/79.5 | Fatty oxidation complex alpha subunit | Appeared | Appeared |
21 | SdhA | Gel match | P10444 | 5.74/63.7 | Succinate dehydrogenase flavoprotein subunit | — | — |
22 | OppA | Gel match | P23843 | 5.93/56.1 | Periplasmic oligopeptide-binding protein | | — |
23 | TrpD | Gel match | P00904 | 6.08/55.9 | Anthranilate synthase component II; Anthranilate | — | — |
24 | GuaB (GuaR) | Gel match | P06981 | 6.01/55.0 | Inosine--monophosphate
dehydrogenase | | |
25 | AtpA | Gel match | P00822 | 5.84/53.1 | ATP synthase alpha chain | — | |
26 | DppA | Gel match | P23847 | 5.69/52.1 | Periplasmic dipeptide transport protein | — | — |
27 | GlyA | Gel match | P00477 | 6.04/45.9 5.94/46.1 | Serine hydroxymethyltransferase (Serine methylase) | — | — |
28 | CarA (PyrA) | Gel match | P00907 | 5.91/44.0 | Carbamoyl-phosphate synthase small chain | — | — |
29 | Unknown | MS/MS | — | — | — | Disappeared | — |
30 | Unknown | MS/MS | — | — | — | Disappeared | Disappeared |
31 | FadA | MS/MS | P21151 | 6.31/40.9 | Fatty oxidation complex beta subunit | Appeared | Appeared |
32 | Fba | Gel match | P11604 | 5.55/40.6 | Fructose-bisphosphate aldolase class II | — | — |
33 | SerC (PdxF) | Gel match | P23721 | 5.34/40.2 | Phosphoserine aminotransferase | | |
34 | SucC | Gel match | P07460 | 5.30/42.3 | Succinyl-CoA synthetase beta
chain | | |
35 | LivJ | MS/MS | P02917 | 5.28/41.9 | Leu/Ile/Val-binding protein | — | — |
36 | Pgk | Gel match | P11665 | 5.07/41.9 5.02/41.7 | Phosphoglycerate kinase | — | |
37 | MalE | MS/MS | P02928 | 5.08/41.1 | Maltose-binding periplasmic protein | Appeared | Appeared |
38 | LivK | Gel match | P04816 | 5.00/41.4 | Leucine-specific binding protein | — | — |
39 | RfaD (HtrM) | Gel match | P17963 | 4.85/36.8 | ADP-L-glycero-D-manno-heptose-6-epimerase | — | — |
40 | PotD | Gel match | P23861 | 4.77/35.8 | Spermidine/putrescine-binding periplasmic protein | — | — |
41 | TalB | Gel match | P30148 | 5.01/35.8 | Transaldolase B | — | |
42 | Tsf (EF-Ts) | Gel match | P02997 | 5.15/33.6 | Elongation factor Ts (EF-Ts) | — | |
43 | Mdh | MS/MS | P06994 | 5.55/35.5 | Malate
dehydrogenase | | — |
44 | CysK | Gel match | P11096 | 5.81/36.0 | Cysteine synthase A | — | — |
45 | ManX (PtsL) | Gel match | P08186 | 5.17/26.1 | PTS system, mannose-specific IIAB component | | — |
46 | Unknown | MS/MS | — | — | — | 2 | 3 |
47 | AroG | Gel match | P00886 | 6.12/39.4 | Phospho-2-dehydro-3-deoxyheptonate
aldolase | 2 | |
48 | Sbp | Gel match | P06997 | 6.49/49.5 | Sulfate-binding protein | 2 | |
49 | GapA | Gel match | P06977 | 6.58/36.3 | Glyceraldehyde 3-phosphate dehydrogenase A | — | — |
50 | PyrB | Gel
match | P00479 | 6.13/35.3 | Aspartate carbamoyltransferase catalytic chain | — | — |
51 | FkpA | Gel
match | P45523 | 7.08/33.2 | FKBP-type peptidyl-prolyl cis-trans isomerase FkpA | — | — |
52 | SucD | MS/MS | P07459 | 6.31/29.6 | Succinyl-CoA synthetase alpha chain | | |
53 | GapA | MS/MS | P06977 | 6.58/23.0 | No. 49 fragment | Disappeared | Disappeared |
54 | GlnH | MS/MS | P10344 | 6.87/24.9 | Glutamine-binding periplasmic protein | — | — |
55 | SodA | Gel match | P00448 | 6.44/22.9 | Superoxide dismutase [Mn] (MnSOD) | — | — |
56 | RbsB | MS/MS | P02925 | 5.92/29.1 | D-ribose-binding periplasmic protein | Appeared | Appeared |
57 | Udp | Gel match | P12758 | 5.86/27.9 | Uridine phosphorylase (UDRPase) | Appeared | Appeared |
58 | YadK | Gel match | P37016 | 5.55/28.4 | Protein YadK | — | — |
59 | TpiA (Tpi) | Gel match | P04790 | 5.57/26.9 | Triosephosphate isomerase | — | — |
60 | Cdd | MS/MS | P13652 | 5.08/31.5 | Cytidine
deaminase | Appeared | Appeared |
| | MS/MS | | 5.42/31.5 | | Appeared | Appeared |
61 | TrpA | Gel match | P00928 | 5.30/28.7 | Tryptophan synthase alpha chain | — | — |
62 | SspA (Ssp) | Gel match | P05838 | 5.24/26.6 | Stringent starvation protein A | — | — |
63 | HisJ | Gel match | P39182 | 5.05/28.6 | Histidine-binding periplasmic protein | — | — |
64 | FliY | Gel match | P39174 | 5.01/26.2 5.11/25.8 | Cystine-binding periplasmic protein | | 3 |
65 | HdhA (HsdH) | Gel match | P25529 | 5.17/25.0 | 7-alpha-hydroxysteroid dehydrogenase | | |
66 | Upp (UraP) | Gel match | P25532 | 5.29/23.8 | Uracil phosphoribosyltransferase | — | — |
67 | GrpE | Gel match | P09372 | 4.68/25.5 | GrpE protein (HSP-70 cofactor) | — | 2 |
68 | AccB (FabE) | Gel match | P02905 | 4.57/22.0 | Biotin carboxyl carrier protein of acetyl-CoA carboxylase | 2 | 2 |
69 | YfdX | MS/MS | P76520 | 5.38/23.0 | Protein yfdX | Disappeared | Disappeared |
70 | AhpC | Gel match | P26427 | 5.01/21.5 | Alkyl hydroperoxide reductase C22 protein | | |
71 | Crr | Gel match | P08837 | 4.57/20.0
4.68/18.9 | PTS system, glucose-specific IIA component | — | — |
72 | DksA | Gel match | P18274 | 4.90/18.7 | DnaK suppressor protein | — | |
73 | AroK | Gel match | P24167 | 5.30/17.9 | Shikimate kinase I | — | — |
74 | SodB | Gel match | P09157 | 5.53/22.1 | Superoxide dismutase [Fe] | 2 | |
75 | PpiB | Gel match | P23869 | 5.51/17.7 | Peptidyl-prolyl cis-trans isomerase B | — | |
76 | RplI | Gel match | P02418 | 6.20/19.8
6.17/15.7 | 50S ribosomal protein L9 | | |
77 | YbdQ | Gel match | P39177 | 6.08/15.5 | Unknown protein from 2D-page | — | — |
78 | RbfA | Gel match | P09170 | 6.00/15.6 | Ribosome-binding factor A | — | — |
79 | RplU | Gel match | P02422 | 6.71/10.3 | 50S ribosomal protein L21 | 2 | 3 |
80 | Hns | Gel match | P08936 | 5.45/15.6 | DNA-binding protein H-NS (Histone-like protein HLP-II) | — | |
81 | Ndk | Gel match | P24233 | 5.59/15.2 | Nucleoside diphosphate kinase (NDP kinase) | — | — |
82 | AtpC | Gel match | P00832 | 5.48/14.8 | ATP synthase epsilon chain | — | — |
83 | RpsF | Gel match | P02358 | 5.31/15.8
5.15/15.8
5.26/15.8 | 30S ribosomal protein S6 | — | — |
84 | Bcp | Gel match | P23480 | 5.02/15.8 | Bacterioferritin comigratory protein | | 2 |
85 | GreA | Gel
match | P21346 | 4.68/15.9 | Transcription elongation factor GreA | 2 | Disappeared |
86 | GroES (MopB) | Gel match | P05380 | 5.15/15.6 | 10 kDa chaperonin (GroES protein) | — | — |
87 | YfiD | MS/MS | P33633 | 5.09/14.3 | Protein YfiD | — | — |
88 | UspA | Gel match | P28242 | 5.14/15.1 | Universal stress protein A | — | — |
89 | YjgF | Gel
match | P39330 | 5.29/13.0 | Protein YjgF | | |
90 | TrxA (TsnC) | Gel match | P00274 | 4.67/11.5 | Thioredoxin 1 | — | — |
91 | HdeB | Gel match | P26605 | 4.85/11.2 | Protein HdeB (10K-L protein) | — | 3 |
92 | YccU | MS/MS | P75874 | 6.72/14.7 | Protein YccU; Predicted CoA-binding protein | Appeared | Appeared |
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