Characterization for Binding Complex Formation with Site-Directly Immobilized Antibodies Enhancing Detection Capability of Cardiac Troponin I
Figure 1
Affinity chromatography for the isolation of Fab from the antibody fragment mixture digested by Ficin protease and its identification. After loading the cleaved products on a protein A column, the unbound fractions were collected and the bound were eluted by shifting the medium pH. Each fraction was analyzed for total antibody and for that with Fc, which showed that the unbound included Fab and the bound consisted of fragments containing Fc (a). Furthermore, SDS-PAGE under non-reducing conditions confirmed that Fab was indeed the main component in the unbound portion. (b).
