Characterization for Binding Complex Formation with Site-Directly Immobilized Antibodies Enhancing Detection Capability of Cardiac Troponin I
Figure 3
Dose-response curves for binding between each type of biotinylated antibody and cTnI at low ( pmol/mL addition) and high ( pmol/mL) surface densities of the respective antibody. The antibodies were immobilized via biotin-streptavidin linkage and used as the capture for carrying out sandwich enzyme immunoassays for cTnI. The signals produced from the assays were plotted against the analyte concentration. The mean of triplicate determinations for each measurement and the corresponding standard deviations were indicated.
