Characterization for Binding Complex Formation with Site-Directly Immobilized Antibodies Enhancing Detection Capability of Cardiac Troponin I
Figure 6
Equilibrium binding models of immobilized antibodies at solid surfaces. The models were devised on the basis of the variation patterns of the reactive antibody density (refer to Figure 4) and binding affinity (refer to Figure 5) against the antibody concentration for four different biotinylated preparations (see text for details). The major factor controlling the antigen-antibody complex formation seemed to be molecular orientation at a low antibody density (left) and accessibility at a high density (right).
