A Multisampling Reporter System for Monitoring MicroRNA Activity in the Same Population of Cells

Figure 1

Characterization of the multisampling reporter system. HEK293T cells were grown in 6-well plates to 70% confluency, then some were left untreated and others were transfected with 0.5 𝜇g of pMLuc or pSEAP2-Control for 24 hours. (a) An aliquot of the medium was assayed for MLuc (left) or SEAP (right). (b) The culture medium was collected and incubated at 37^∘C in a 5% CO₂ humidified chamber and aliquots were withdrawn at different time points and assayed for MLuc (left) or SEAP (right). (c) Medium samples were frozen at −20^∘C in separate Eppendorf tubes, and thawed at the indicated times, and assayed. The data presented are the mean ± SD of triplicates.

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