A Multisampling Reporter System for Monitoring MicroRNA Activity in the Same Population of Cells
Figure 2
Reporting ability of the multisampling reporter system. HEK293T cells were grown in 6-well plates to 70% confluency. (a) The cells were cotransfected with 0.5 g of pSEAP2-Control, 0.5 g of pMLuc or pMLuc-UTR, and 30 nM pre-miR-16 or NC miR. The medium was replaced with fresh medium at different time points after transfection, and an aliquot of medium was withdrawn 2 hours later and assayed for MLuc and SEAP. (b) The cells were cotransfected with 0.5 g of pMLuc-UTR and pSEAP2-Control, together with different concentration of pre-miR-16 (0–35 nM) and NC miR (35–0 nM), then, 24 hours later, an aliquot of medium was withdrawn from each culture medium and assayed. The data presented are the mean ± SD of triplicates.
