Experimental Study of the Effects of Marrow Mesenchymal Stem Cells Transfected with Hypoxia-Inducible Factor-1𝛼 Gene

Figure 11

Transfecton efficency = (The number of cells expressing green florescence/The number of cells expressing blue florescence) × 100%, and the average value obtained from 3∼4 visual field. (a) The cells type and Hoechst33342 stained nucleus that showed light blue under optical microscope. (b) Hoechst33342 can directly permeate the normal membrane and dye the DNA of viable cells, which showed the nucleus expressed blue fluorescence. (c) The cells transfected with HIF-1𝛼-pcDNA_3.1/eGFP, showed green fluorescence with whole cell type.

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