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147946.fig.003b
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Figure 3: Cx45 immunofluorescent stainings indicate amplified expression in the ischemic lesion (b) compared to the intact area (a). Inset pictures of (c) and (d) represent double immunofluorescent staining with Cx45 (green) and MAP2 (red). Representative pictures in second row show the extracted area as yellow region of the double immunofluorescent staining from intact (c) and ischemic area (d). The yellow region which stands for Cx45 immunopositive area colocalized with MAP2 shows increased expression in the ischemic periphery. Inset pictures of (e) and (f) represent double immunofluorescent staining with Cx45 (green) and GFAP (red). Representative pictures in third row show the extracted area as yellow region of the double immunofluorescent staining from intact (e) and ischemic area (f). The yellow region which stands for Cx45 immunopositive area colocalized with GFAP shows no difference of expression between intact and ischemic area. Black bars in graph (g) indicate the average counts of protein expressions. The Cx45 expression was significantly increased in the ischemic area compared to the intact area. The coexpression of Cx45 and MAP2 was significantly increased in the ischemic lesion as compared to the intact area. However, no statistical alteration was observed in the amount of coexpression of Cx45 and GFAP between intact and ischemic regions. Scale bar indicates 50  m. **: .