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Figure 4: Cx43 immunofluorescent stainings indicate amplified expression in the ischemic lesion (b) compared to the intact area (a). Inset pictures of (c) and (d) represent double immunofluorescent staining with Cx43 (green) and MAP2 (red). Representative pictures in second row show the extracted area as yellow region of the double immunofluorescent staining from intact (c) and ischemic area (d). The yellow region which stands for Cx43 immunopositive area colocalized with MAP2 shows amplified expression in the ischemic periphery. Inset pictures of (e) and (f) represent double immunofluorescent staining with Cx43 (green) and GFAP (red). Representative pictures in third row show the extracted area as yellow region of the double immunofluorescent staining from intact (e) and ischemic area (f). The yellow region which stands for Cx43 immunopositive area colocalized with GFAP also shows increased expression level in ischemic lesion as compared to intact area. Black bars in graph (g) indicate the average counts of protein expressions. The Cx43 expression alone and the amount of coexpression with Cx43 or GFAP were significantly increased in the ischemic lesion compared to the intact area. The coexpression of Cx43 and MAP2 was increased in ischemic lesion compared to intact area (†: ). Scale bar indicates 50  m. **: , *: .