Figure 5: Cx26 immunofluorescent stainings show similar finding between intact area (a) and ischemic lesion (b). Inset pictures represent double immunofluorescent staining with Cx26 (green) and MAP2 (red) in (c) and (d) and with Cx26 (green) and GFAP (red) in (e) and (f). Representative pictures in second row show the extracted area as yellow region of the double immunofluorescent staining from intact (c) and ischemic area (d). The yellow region which stands for Cx26 immunopositive area colocalized with MAP2 shows the same amount of expression. The yellow region which stands for Cx26 immunopositive area colocalized with GFAP shows no difference of expression between intact and ischemic area. Black bars in graph (g) indicate the average counts of protein expressions. The Cx26 expression was not changed in the ischemic lesion as compared to the intact area. No statistical alteration was observed in the amount of coexpression of Cx26 and MAP2 nor Cx26 and GFAP between intact and ischemic regions. Scale bar indicates 50 m.