Isolation of Osteosarcoma-Associated Human Antibodies from a Combinatorial Fab Phage Display Library
Figure 1
Selection of Fab clones able to recognize osteosarcoma cell protein extracts. Protein extracts from Saos-2 cells (horizontal line bars, panel (a)), U2-OS cells (horizontal lines, panel (b)), and h FOB 1.19 (normal human osteoblast, black bars, both panels) were used to coat a 96-well plate. E. coli supernatants from the N system (panel (a)) or the O system (panel (b)) Fab clones were added to the plate. After washing, the bound Fabs were detected using rabbit anti-HA antibody followed by an HRP-conjugated anti-rabbit antibody. The experiments were performed in triplicate. The Fabs were also added to HSA- (human serum albumin) coated wells (diagonal line bars, both panels) as a negative control. Supernatant from an empty vector E. coli culture was also used as negative control (pComb). The absorbances were measured at 405 nm.
