Isolation of Osteosarcoma-Associated Human Antibodies from a Combinatorial Fab Phage Display Library

<table>Detection of osteosarcoma antigens by selected Fabs. A western blot using five selected purified Fabs was performed. Protein extracts from U2-OS cells (lane 1) and normal human fibroblasts (lane 2) were transferred to nitrocellulose membranes. The membranes were probed with the O-F4, N-H7, N-H10, N-E8, and O-G11 Fabs. For loading comparison, an anti-alpha tubulin antibody was also used.</table>

BioMed Research International

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Figure 3

Figure 3: Isolation of Osteosarcoma-Associated Human Antibodies from a Combinatorial Fab Phage Display Library 