Isolation of Osteosarcoma-Associated Human Antibodies from a Combinatorial Fab Phage Display Library
Figure 5
Indirect immunofluorescence staining and immunohistochemistry were performed with the selected Fabs. Methanol-fixed fibroblasts ((a) and (c)) or U2-OS osteosarcoma cells ((b) and (d)) were stained with Fab N-H7 ((a) and (b)) or Fab O-G11 ((c) and (d)). Fab O-G11 was also used to stain to normal bone (e) (200x) and osteosarcoma tissue (f) (400x) sections. The osteoblasts in normal bone are arranged in lines; the empty region corresponds to bone trabecules dislocated during washing. These results are representative from four different osteosarcoma cases analyzed.
