p,p^′-DDE Induces Apoptosis of Rat Sertoli Cells via a FasL-Dependent Pathway

Figure 3

Representative plots of PI-Annexin staining of Sertoli cells treated with p,p^′-DDE with or without NAC. Sertoli cells were incubated with various concentrations of p,p^′-DDE (10, 30 or 50 μM) for 24 hours. In other experiment, Sertoli cells were preincubated with 300 μM NAC for 1 hour and followed by incubation with 50 μM p,p^′-DDE for 24 hours. Then cell apoptosis was tested with flow cytometric analysis. (a)–(e) represented as treatment of 0.3% DMSO (a), 10 or 30 μM p,p^′-DDE (b)–(c), 50 μM p,p^′-DDE without or with NAC (d)–(e). (f) The apoptotic rate showed that 30 or 50 μM p,p^′-DDE could induce apoptotic death of Sertoli cells blocked by NAC. Data are presented as mean ± SD of three independent experiments performed in triplicate. Significant difference is (^∗)𝑃<.05, compared with the control group and (^#)𝑃<.05, compared with 50 μM p, p^′-DDE group.

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