Construction of a Recombinant Eukaryotic Expression Plasmid Containing Human Calcitonin Gene and Its Expression in NIH3T3 Cells

Table 1

The number of TRAP-positive multinucleate cells when osteoclasts were cocultured in vitro with pCDNA3.0-Ig-hCT-transfected, and nontransfected, pCDNA3.0-transfected NIH3T3 cells, or nontransfected NIH3T3 cells plus sCT.

Group	Number of TRAP-positive multinucleate cells (means ± SD)
Nontransfected group	18.3±3.2
pCDNA3.0-transfected group	16.9±2.7
pCDNA3.0-Ig𝜅-hCT-transfected group	12.7±1.9 *
Nontransfected group plus sCT	9.5±2.4^**

* 𝑃<.01, compared with nontransfected group and pCDNA3.0-transfected group. ^**𝑃<.01, compared with pCDNA3.0-transfected group.