The pol3-t Hyperrecombination Phenotype and DNA Damage-Induced Recombination in Saccharomyces cerevisiae Is RAD50 Dependent

Table 1

Saccharomyces cerevisiae strains.

Name	Parent strain	Genotype	Source
RSY6		MATa  ura3-52 leu2-3,112 trp5-27 ade2-40 ilv1-92 arg4-3 his3 ▵5^’-pRS6-his3 ▵3^’	[13]
YR50-1	RSY6	MATa  ura3-52 leu2-3,112 trp5-27 ade2-40 ilv1-92 arg4-3 his3∆5^’-pRS6-his3∆3^’, rad50::hisG	Schiestl collection
AGY30	RSY6	MATa  ura3-52 leu2-3,112 trp5-27 ade2-40 ilv1-92 arg4-3 his3∆5^’-pRS6-his3∆3^’ pol3-t	[14]
YMG1	RSY6	MATa  ura3-52 leu2-3,112 trp5-27 ade2-40 ilv1-92 arg4-3 his3∆ 5^’-pRS6-his3∆ 3^’, MAG1::hisG	Schiestl collection
AGY40	YMG1	MATa  ura3-52 leu2-3,112 trp5-27 ade2-40 ilv1-92 arg4-3 his3∆ 5^’-pRS6-his3∆ 3^’, MAG1::hisG, pol3-t	This study
AGY34	YR50-1	MATa  ura3-52 leu2-3,112 trp5-27 ade2-40 ilv1-92 arg4-3 his3∆ 5^’-pRS6-his3∆3^’ pol3-t, rad50::hisG	This study
RSY12		MATa  leu2-3,112 his3-11,15 URA3::HIS3	[15]
YR50-12	RSY12	MATa  leu2-3,112 his3-11,15 URA3::HIS3, rad50::LEU2	[16]
AGY38	RSY12	MATa  leu2-3,112 his3-11,15 URA3::HIS3,pol3-t	[17]
AGY39	YR50-12	MATa  leu2-3,112 his3-11,15 URA3::HIS3-pol3-t, rad50::LEU2	This study