The pol3-t Hyperrecombination Phenotype and DNA Damage-Induced Recombination in Saccharomyces cerevisiae Is RAD50 Dependent

Table 2

Effect of RAD50 on the hyperrecombination phenotype of pol3-t.

Strain	Genotype	Intrachromosomal recombination (×10^−4)
		25^∘C	30^∘C
RSY6	RAD, POL3	2.49 ± 1.55	2.70 ± 0.89
AGY30	RAD, pol3-t	36.8 ± 12.66^***	86.52 ± 10.57^***
YR50-1	rad50Δ, POL3	1.92 ± 0.67	1.53 ± 0.96
AGY34	rad50Δ, pol3-t	2.72 ± 1.02	1.86 ± 1.07

Single colonies of each strain were inoculated in SC-LEU and incubated for 24 hour at 25^∘ or 30^∘C. Then, cells were washed and plated to determine the frequency of intrachromosomal recombination as described in the Materials and Methods. Results are the mean of 6 independent experiments ± standard deviation. ^∗∗∗𝑃<.001.