The pol3-t Hyperrecombination Phenotype and DNA Damage-Induced Recombination in Saccharomyces cerevisiae Is RAD50 Dependent

Table 3

Effect of pol3-t on homologous integration in Saccharomyces cerevisiae. Homologous gene targeting was measured by transformation of the plasmid pJZ102 digested with EcoRI-HindIII to release a URA3 fragment that was flanked by the 5^’ and 3^’ ends of LYS2 gene. Only URA3lys2 colonies were counted as homologous integrants. In parentheses, the total URA3lys2 colonies counted are reported. Data are reported as mean of at least 6 independent experiments ± standard deviation.

Strain	Integration events/𝜇g DNA per 10^4 transformed cells
RSY12 (POL3RAD50)	263.9 ± 79.3 (4310)
AGY38 (pol3-t)	2936.8 ± 769.8 (22247)^∗∗∗(a)
YR50-12 (rad50Δ)	434.6 ± 104.1 (7196)
AGY3 (rad50Δpol3-t)	2780.4 ± 445.5 (17416)^∗∗∗(a)(b)

^∗∗∗𝑃<.005
^(a)Data were statistically compared to RSY12
^(b)Data were statistically compared to rad50Δ.