BioMed Research International

Research Article

The pol3-t Hyperrecombination Phenotype and DNA Damage-Induced Recombination in Saccharomyces cerevisiae Is RAD50 Dependent

Table 4

Effect of pol3-t on MMS-induced intrachromosomal recombination in RAD ⁺ and strains. Data corresponding to RSY6 and AGY30 were previously published [14]. Results are reported as the mean of five or more independent experiments standard deviation. All strains are isogenic. The probabilities refer to the comparison between the exposure and the untreated control.


Strain	MMS	% Survival	Intrachromosomal recombination
Strain	(g/ml)	% Survival	()

RSY6 (POL3 RAD50)	0	100	4.3 1.0
	10	89 15	9.3 1.
	100	92 8	39 6.
	200	89 15	51 1
	500	48 19	102 2

AGY30 (pol3-t)	0	100	17 4.6
	10	100 0.5	14 8.4
	100	90 0.5	30 4.
	200	45 1.5	60 2
	500	0.8 0.6	117 2.

YR50-1 (rad50∆)	0	100	1.8 0.6
	1	66 11	1.6 0.5
	10	54 16	2.4 0.8
	50	19 2.8	2.3 0.2
	100	3.9 0.5	3.6 0.
	200	0.4 0.2	4.2 0.

AGY34 (rad50∆pol3-t)	0	100	1.8 0.2
	1	84 22	1.7 0.1
	10	58 14	1.7 0.6
	50	37 8.5	2.2 0.4
	100	7.2 2	3.5 0.
	200	0.8 0.7	12.8 1.

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