Modular Cre/lox System and Genetic Therapeutics for Colorectal Cancer
Figure 3
Wnt-specific SW620 cell kill. SW620 cells in a 24-well plate were transfected with 0.05 g of pGL3-Ctl, 0.5 g of STOP-FADD, and (a) 0.05 g of TOP/FOPTK-CRE, (b) 0.0067 g of TOP/FOPCMV-CRE, (c) 0.1 g of TOP/FOPFos-CRE, or (d) 0.15 g of TOP/FOPTATA-CRE, with equivalent amounts of the 0-CRE control expression vector per well, and untreated or treated with 5 mM butyrate, and assayed for luciferase expression 48 hours later. Luciferase readings were normalized to protein concentration determined for each sample. The input plasmids for each experiment are listed at the top of each graph. Results are from three independent experiments. Bars indicate standard deviations, reproduced as per copyright agreement from [33].
