Efficient siRNA Delivery by the Cationic Liposome DOTAP in Human Hematopoietic Stem Cells Differentiating into Dendritic Cells

Figure 3

Expression of phenotypic markers throughout HSC differentiation into immunogenic dendritic cells in presence of multiple, DOTAP-mediated transfection rounds. HSCs were subjected to differentiation agents, and then immediately split into two parallel cultures, one of which was maintained in the absence of manipulations, while the other was subjected to DOTAP-based, scrambled siRNA transfection rounds. Phenotypic antigens typically modulated during HSC differentiation into immunogenic dendritic cells were analyzed in HSCs at day 0 (white bars), day 3 (grey bars), and day 14 (black bars). In all instances, analyses were performed through a FACScan flow cytometer using monoclonal antibodies labeled with fluorescein isothiocyanate or phycoerythrin. Data shown are representative of triplicate determinations.