Molecularly Characterised Xenograft Tumour Mouse Models: Valuable Tools for Evaluation of New Therapeutic Strategies for Secondary Liver Cancers

Table 1

Sequences amplified on TaqMan low density arrays.

Gene	Gene name	Classification	TaqMan assay ID^a
BCL2	B-cell CLL/lymphoma 2	Inhibition of apoptosis	Hs00153350_m1
CCND1	Cyclin D1	Kinase activator, cell cycle control, proliferation	Hs00277039_m1
CDC25B	Cell division cycle 25B	Protein phosphatase, cell proliferation	Hs00244740_m1
CDKN1B	Cyclin-dependent kinase inhibitor 1B (p27, Kip1)	Cell cycle control, tumour suppressor	Hs00153277_m1
CTNNB1	Catenin (cadherin-associated protein), beta 1, 88 kDa	Cytoskeletal protein, cell adhesion, oncogenesis	Hs00170025_m1
EGFR	Epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog)	Cell cycle control, proliferation, oncogenesis	Hs00193306_m1
ERBB2	v-erb-b2 erythroblastic leukemia viral oncogene homolog 2	Protein kinase receptor, oncogenesis, cell cycle control	Hs00170433_m1
ETV4	ets variant gene 4 (E1A enhancer binding protein, E1AF)	Transcription factor, oncogenesis, cell motility	Hs00385910_m1
IL6	Interleukin 6 (interferon, beta 2)	Chemokine, inhibition of apoptosis	Hs00174131_m1
IL6R	Interleukin 6 receptor	Cell proliferation, immunity and defense	Hs00169842_m1
IL8	Interleukin 8	Angiogenesis, cell proliferation/differentiation	Hs00174103_m1
KRAS	v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog	Small GTPase, cell proliferation/differentiation	Hs00270666_m1
MET	Met proto-oncogene (hepatocyte growth factor receptor)	Protein kinase receptor, oncogenesis	Hs00179845_m1
MMP1	Matrix metalloproteinase 1 (interstitial collagenase)	Metalloprotease, extracellular matrix break down	Hs00233958_m1
MMP11	Matrix metalloproteinase 11 (stromelysin 3)	Metalloprotease, inhibition of apoptosis	Hs00171829_m1
MYC	v-myc myelocytomatosis viral oncogene homolog	Oncogene, cell cycle control	Hs00153408_m1
PTGS2	Prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase, Cox-2)	Oxidoreductase, lipid metabolism, deregulated in epithelial tumours	Hs00153133_m1
SERPINB5	Serine proteinase inhibitor, clade B (ovalbumin), member 5	Proteinase inhibitor, oncogenesis	Hs00184728_m1
VEGFA	Vascular endothelial growth factor	Growth factor, angiogenesis	Hs00173626_m1
VEGFC	Vascular endothelial growth factor C	Cell proliferation and differentiation	Hs00153458_m1
WNT1	Wingless-type MMTV integration site family, member 1	Signalling molecule, oncogenesis	Hs00180529_m1
rRNA18S^b	18S ribosomal RNA	Eukaryotic ribosomal RNA gene, obligatory control	4342379-18S
GUSB^b	Glucuronidase, beta	Galactosidase, carbohydrate metabolism	Hs99999908_m1
ACTB^b	Actin, beta	Cytoskeletal protein	Hs99999903_m1

^am1 indicates that the TaqMan minor groove binding probe spans an exon junction and will not detect genomic DNA;
^bendogenous control genes shown in a pilot study (TaqMan human endogenous control plate) to be equally expressed in all samples investigated.