Research Article

Molecularly Characterised Xenograft Tumour Mouse Models: Valuable Tools for Evaluation of New Therapeutic Strategies for Secondary Liver Cancers

Table 4

Relative differences in gene expression levels (n-fold) of original tumour samples compared to the corresponding xenograft tumour. Indicated values represent the mean of three measurements including the calculated standard deviation. Ratios were calculated after normalisation of individual RNA amounts to a standard reference RNA. Values indicating differences higher than 2.5-fold are printed in bold. Gene symbols correspond with Table 1. n.d.: not determinable, Ct values obtained with cDNA derived either from the xenograft (#) or from both tumour samples were below threshold (>39).

AKH10AKH23KFJ6KFJ9KFJ10KFJ12KFJ18KFJ21

BCL20.35  ±  0.06 0.37  ±  0.09
CCND1
CDC25B 2.70  ±  0.44
CDKN1B
CTNNB1
EGFR
ERBB2
ETV4
IL6 0.26  ±  0.04n.d.#0.30  ±  0.04n.d.#n.d.#n.d.#n.d.#
IL6R4.86  ±  1.35 n.d.# 18.33  ±  1.984.41  ±  0.3
IL8 2.65  ±  1.018.46  ±  5.6712.81  ±  3.4323.46  ±  6.7419.39  ±  4.4334.84  ±  3.42112.68  ±  23.33
KRAS2
MET
MMP1 n.d.# n.d. n.d. n.d.#
MMP1115.62  ±  6.1022.13  ±  5.085.66  ±  0.21n.d.#8.33  ±  1.683.98  ±  1.2413.72  ±  2.4310.88  ±  0.42
MYC
PTGS2 n.d.#n.d.#n.d.#n.d.#n.d.#n.d.#
SERPINB5n.d.17.66  ±  4.92 0.38  ±  0.05
VEGFA 0.17  ±  0.01 2.78  ±  0.32
VEGFC n.d.#n.d.#n.d.#n.d.#n.d.#n.d.#n.d.#
WNT1n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.