Dermaseptins and Magainins: Antimicrobial Peptides from Frogs' Skin—New Sources for a Promising Spermicides Microbicides—A Mini Review

Figure 2

Effect of spermicidal dermaseptins on sperm acrosome and flagellar plasma membrane integrity. Motile sperm were preincubated in the presence of spermicide solution (EC100 = 25 𝜇g/mL) of K4S4 and was double labelled with Fluorescein Isothiocyanate-conjugated-Pisum sativum Agglutinin for acrosome labelling (FITC/green) and mouse antitubulin antibody for the flagellum staining (CY3/red). The sperm heads exhibited a “dotted” green fluorescence attesting for the presence of an acrosome. At the flagellar level, we observed a loss of membrane integrity since the anti tubulin antibodies have reached their axonemal microtubule target [44].