A Significant Increase of RNAi Efficiency in Human Cells by the CMV Enhancer with a tRNAlys Promoter

Figure 1

Map of construscts used in this work. SP1, SP2 and SP3 plasmids derived from the pSiler plasmid can express shRNA1, shRNA2, and shRNA3, respectively. EP1, EP2 and EP3 plasmids derived from the pExsiler plasmid can express shRNA1, shRNA2, and shRNA3, respectively. UP3 plasmid derived from pBS/U6 plasmid can express shRNA3. As control, ET plasmid derived from the pExsiler plasmid can express no shRNA. pEGFP(N2)-Pokemon derived from pEGFP-N2 vector can express Pokemon-GFP fusion protein.