Research Article
A Significant Increase of RNAi Efficiency in Human Cells by the CMV Enhancer with a tRNAlys Promoter
Table 1
Sequences of oilgonucleotides used in the research.
| shRNA | Sequences of oilgonucleotides | Target site on pokemon mRNA (GenBank accession number NM_01 5898) |
| 1 | F1: -ccgtgtacgagatcgacttcttcaagagagaagtcgatctcgtacacgtttttt- | 383–401 nt | | R1: -ccggggcacatgctctagctgaagaagttctctcttcagctagagcatgtgcaaaaaatcga- | | 2 | F2: -ccggcttagacttctatgggttcaagagacccatagaagtctaagccgtttttt- | 809–827 nt | | R2: -ccggggccgaatctgaagatacccaagttctctgggtatcttcagattcggcaaaaaatcga- | | 3 | F3: -cgggcgtcatggactactacttcaagagagtagtagtccatgacgccctttttt- | 1202–1200 nt | | R3: -ccgggcccgcagtacctgatgatgaagttctctcatcatcaggtactgcgggaaaaaatcga- | |
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shRNA encoding cassettes 1–3 were formed by annealing two corresponding oligonucleotides, respectively. F stands for forward and R for reverse.
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