YQ36: A Novel Bisindolylmaleimide Analogue Induces KB/VCR Cell Death
Figure 4
YQ36 caused apoptosis in KB/VCR cells in vitro. (a) YQ36 induced a characteristic fragmentation of DNA in KB/VCR cells (10 M, 6–24 hours). (b) The result of measuring caspase-3/7 activity indicated YQ36 induced KB/VCR cells apoptosis in time-dependent manner. The error bars represent standard deviation and **represent versus control. (c) Caspase activation and mitochondrial pathway involved in YQ36-induced apoptosis. Cells were treated with YQ36 (10 M) for 6, 12, and 24 hours, and whole-cell lysates were collected and immunoblotted with indicated antibodies. (d) The expressions of antiapoptotic proteins were decreased in KB/VCR cells (10 M YQ36, 6–24 hours). (e) The protein expression of Bax in mitochondrial fraction after treated with YQ36 (10 M) for 6, 12, and 24 hours, and the HSP60 was used as a housekeeper for mitochondrial fraction. (f) Densitometric analysis of expression of Bcl-2 and Bax relative to the control.
