Review Article
DNA, RNA, and Protein Extraction: The Past and The Present
Table 1
Typical biological interactions used in affinity chromatography [
42].
| Types of ligand | Target molecules |
| Enzyme | Substrate analogue, inhibitor, cofactor | Antibody | Antigen, virus, cell | Lectin | Polysaccharide, glycoprotein, cell surface receptor, cell | Nucleic acid | Complementary base sequence, histones, nucleic acid polymerase, nucleic acid binding protein | Hormone, vitamin | Receptor, carrier protein | Glutathione | Glutathione-S-transferase or GST fusion proteins | Metal ions | Poly (his) fusion proteins, native proteins with histidine, cysteine and/ortryptophan residues on their surfaces |
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